2dob

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[[Image:2dob.gif|left|200px]]
[[Image:2dob.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2dob |SIZE=350|CAPTION= <scene name='initialview01'>2dob</scene>, resolution 2.00&Aring;
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The line below this paragraph, containing "STRUCTURE_2dob", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= PSAP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-->
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|DOMAIN=
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{{STRUCTURE_2dob| PDB=2dob | SCENE= }}
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|RELATEDENTRY=[[1n69|1N69]], [[1m12|1M12]], [[2gtg|2GTG]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dob OCA], [http://www.ebi.ac.uk/pdbsum/2dob PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2dob RCSB]</span>
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}}
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'''Crystal Structure of Human Saposin A'''
'''Crystal Structure of Human Saposin A'''
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[[Category: Ahn, V E.]]
[[Category: Ahn, V E.]]
[[Category: Prive, G G.]]
[[Category: Prive, G G.]]
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[[Category: lipid-binding protein]]
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[[Category: Lipid-binding protein]]
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[[Category: saposin]]
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[[Category: Saposin]]
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[[Category: sphingolipid activator protein]]
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[[Category: Sphingolipid activator protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:52:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:37:19 2008''
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Revision as of 21:52, 3 May 2008

Image:2dob.gif

Template:STRUCTURE 2dob

Crystal Structure of Human Saposin A


Contents

Overview

Saposins A and C are sphingolipid activator proteins required for the lysosomal breakdown of galactosylceramide and glucosylceramide, respectively. The saposins interact with lipids, leading to an enhanced accessibility of the lipid headgroups to their cognate hydrolases. We have determined the crystal structures of human saposins A and C to 2.0 Angstroms and 2.4 Angstroms, respectively, and both reveal the compact, monomeric saposin fold. We confirmed that these two proteins were monomeric in solution at pH 7.0 by analytical centrifugation. However, at pH 4.8, in the presence of the detergent C(8)E(5), saposin A assembled into dimers, while saposin C formed trimers. Saposin B was dimeric under all conditions tested. The self-association of the saposins is likely to be relevant to how these small proteins interact with lipids, membranes, and hydrolase enzymes.

Disease

Known disease associated with this structure: Combined SAP deficiency OMIM:[176801], Gaucher disease, atypical OMIM:[176801], Krabbe disease, atypical OMIM:[176801], Metachromatic leukodystrophy due to SAP-b deficiency OMIM:[176801]

About this Structure

2DOB is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structures of saposins A and C., Ahn VE, Leyko P, Alattia JR, Chen L, Prive GG, Protein Sci. 2006 Aug;15(8):1849-57. Epub 2006 Jul 5. PMID:16823039 Page seeded by OCA on Sun May 4 00:52:12 2008

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OCA

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