2dpe
From Proteopedia
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[[Image:2dpe.gif|left|200px]] | [[Image:2dpe.gif|left|200px]] | ||
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'''Crystal structure of a secretory 40KDA glycoprotein from sheep at 2.0A resolution''' | '''Crystal structure of a secretory 40KDA glycoprotein from sheep at 2.0A resolution''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2DPE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries]. This structure supersedes the now removed PDB entry | + | 2DPE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1r2v 1r2v]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DPE OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Srinivasan, A.]] | [[Category: Srinivasan, A.]] | ||
[[Category: Srivastava, D B.]] | [[Category: Srivastava, D B.]] | ||
| - | [[Category: | + | [[Category: Involution]] |
| - | [[Category: | + | [[Category: Mammary gland secretion]] |
| - | [[Category: | + | [[Category: Signaling protein]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:54:32 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 21:54, 3 May 2008
Crystal structure of a secretory 40KDA glycoprotein from sheep at 2.0A resolution
Overview
A 40kDa glycoprotein from dry secretion of sheep is implicated as a signaling factor and is named as SPS-40. This protein is secreted only during the early phase of involution when the drastic tissue remodeling occurs in the mammary gland. SPS-40 was purified from sheep dry secretions and crystallized using hanging drop vapour diffusion method. The crystals belong to orthorhombic space group P2(1)2(1)2(1) with cell dimensions, a=62.7A, b=66.4A, c=107.5A. The protein was also cloned for the determination of its complete amino acid sequence. The three-dimensional structure of SPS-40 was determined by X-ray crystallographic method at 2.0A resolution. The structure revealed the presence of an N-linked glycan chain at Asn39. The protein adopts a conformation with a classical (beta/alpha)(8)-barrel fold of triosephosphate isomerase (TIM) (residues 1-237 and 310-360) with an insertion of a small (alpha+beta) domain (residues 240-307) similar to that observed in chitinases. However, the Leu substitution for Glu in the consensus catalytic sequence in SPS-40 causes a loss of chitinase activity. Furthermore, the sugar-binding groove in SPS-40 is distorted considerably from the standard chitin-binding site in chitinase enzymes and hence the binding of chitin-like oligosaccharides is considerably hampered. Three surface loops, His188-His197, Phe202-Arg212 and Phe244-Pro260 have exceptionally high values of B-factors (average=70.5A(2)), indicating the presence of a less defined region.
About this Structure
2DPE is a Single protein structure of sequence from Ovis aries. This structure supersedes the now removed PDB entry 1r2v. Full crystallographic information is available from OCA.
Reference
Crystal structure of a secretory signalling glycoprotein from sheep at 2.0A resolution., Srivastava DB, Ethayathulla AS, Kumar J, Singh N, Sharma S, Das U, Srinivasan A, Singh TP, J Struct Biol. 2006 Dec;156(3):505-16. Epub 2006 Jun 8. PMID:16859926 Page seeded by OCA on Sun May 4 00:54:32 2008
