2dps

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{{Structure
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Leucyltransferase Leucyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.6 2.3.2.6] </span>
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|RELATEDENTRY=[[2dpt|2DPT]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dps OCA], [http://www.ebi.ac.uk/pdbsum/2dps PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2dps RCSB]</span>
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'''Structure of Leucyl/phenylalanyl-tRNA-protein transferase'''
'''Structure of Leucyl/phenylalanyl-tRNA-protein transferase'''
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[[Category: Suto, K.]]
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[[Category: Tomita, K.]]
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[[Category: aminoacyl-trna-protein transferase]]
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[[Category: Aminoacyl-trna-protein transferase]]
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Revision as of 21:56, 3 May 2008

Image:2dps.jpg

Template:STRUCTURE 2dps

Structure of Leucyl/phenylalanyl-tRNA-protein transferase


Overview

Eubacterial leucyl/phenylalanyl-tRNA protein transferase (L/F-transferase), encoded by the aat gene, conjugates leucine or phenylalanine to the N-terminal Arg or Lys residue of proteins, using Leu-tRNA(Leu) or Phe-tRNA(Phe) as a substrate. The resulting N-terminal Leu or Phe acts as a degradation signal for the ClpS-ClpAP-mediated N-end rule protein degradation pathway. Here, we present the crystal structures of Escherichia coli L/F-transferase and its complex with an aminoacyl-tRNA analog, puromycin. The C-terminal domain of L/F-transferase consists of the GCN5-related N-acetyltransferase fold, commonly observed in the acetyltransferase superfamily. The p-methoxybenzyl group of puromycin, corresponding to the side chain of Leu or Phe of Leu-tRNA(Leu) or Phe-tRNA(Phe), is accommodated in a highly hydrophobic pocket, with a shape and size suitable for hydrophobic amino-acid residues lacking a branched beta-carbon, such as leucine and phenylalanine. Structure-based mutagenesis of L/F-transferase revealed its substrate specificity. Furthermore, we present a model of the L/F-transferase complex with tRNA and substrate proteins bearing an N-terminal Arg or Lys.

About this Structure

2DPS is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structures of leucyl/phenylalanyl-tRNA-protein transferase and its complex with an aminoacyl-tRNA analog., Suto K, Shimizu Y, Watanabe K, Ueda T, Fukai S, Nureki O, Tomita K, EMBO J. 2006 Dec 13;25(24):5942-50. Epub 2006 Nov 16. PMID:17110926 Page seeded by OCA on Sun May 4 00:56:01 2008

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