2drz
From Proteopedia
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'''Crystal structure of the earthworm lectin C-terminal domain mutant in complex with lactose''' | '''Crystal structure of the earthworm lectin C-terminal domain mutant in complex with lactose''' | ||
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[[Category: Fujimoto, Z.]] | [[Category: Fujimoto, Z.]] | ||
[[Category: Suzuki, R.]] | [[Category: Suzuki, R.]] | ||
| - | [[Category: | + | [[Category: Beta-trefoil fold]] |
| - | [[Category: | + | [[Category: Earthworm lumbricus terrestri]] |
| - | [[Category: | + | [[Category: Galactose]] |
| - | [[Category: | + | [[Category: In vitro evolution]] |
| - | [[Category: | + | [[Category: Sialic acid]] |
| - | [[Category: | + | [[Category: Sugar binding protein]] |
| - | [[Category: | + | [[Category: Sugar complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 01:04:08 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 22:04, 3 May 2008
Crystal structure of the earthworm lectin C-terminal domain mutant in complex with lactose
Overview
Sialic acid (Sia) is a typical terminal sugar, which modifies various types of glycoconjugates commonly found in higher animals. Its regulatory roles in diverse biological phenomena are frequently triggered by interaction with Sia-binding lectins. When using natural Sia-binding lectins as probes, however, there have been practical problems concerning their repertoire and availability. Here, we show a rational creation of a Sia-binding lectin based on the strategy 'natural evolution-mimicry', where Sia-binding lectins are engineered by error-prone PCR from a Gal-binding lectin used as a scaffold protein. After selection with fetuin-agarose using a recently reinforced ribosome display system, one of the evolved mutants SRC showed substantial affinity for alpha2-6Sia, which the parental Gal-binding lectin EW29Ch lacked. SRC was found to have additional practical advantages in productivity and in preservation of affinity for Gal. Thus, the developed novel Sia-recognition protein will contribute as useful tools to sialoglycomics.
About this Structure
2DRZ is a Single protein structure of sequence from Lumbricus terrestris. Full crystallographic information is available from OCA.
Reference
Tailoring a novel sialic acid-binding lectin from a ricin-B chain-like galactose-binding protein by natural evolution-mimicry., Yabe R, Suzuki R, Kuno A, Fujimoto Z, Jigami Y, Hirabayashi J, J Biochem. 2007 Mar;141(3):389-99. Epub 2007 Jan 18. PMID:17234683 Page seeded by OCA on Sun May 4 01:04:08 2008
