This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

5mxg

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Photorhabdus asymbiotica lectin (PHL) in complex with blood group H trisaccharide

Structural highlights

5mxg is a 1 chain structure with sequence from Photorhabdus asymbiotica subsp. asymbiotica ATCC 43949. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2Å
Ligands:	, , , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

C7BLE4_PHOAA

Publication Abstract from PubMed

Photorhabdus asymbiotica is one of the three recognized species of the Photorhabdus genus, which consists of gram-negative bioluminescent bacteria belonging to the family Morganellaceae. These bacteria live in a symbiotic relationship with nematodes from the genus Heterorhabditis, together forming a complex that is highly pathogenic for insects. Unlike other Photorhabdus species, which are strictly entomopathogenic, P. asymbiotica is unique in its ability to act as an emerging human pathogen. Analysis of the P. asymbiotica genome identified a novel fucose-binding lectin designated PHL with a strong sequence similarity to the recently described P. luminescens lectin PLL. Recombinant PHL exhibited high affinity for fucosylated carbohydrates and the unusual disaccharide 3,6-O-Me2-Glcbeta1-4(2,3-O-Me2)Rhaalpha-O-(p-C6H4)-OCH2CH2NH2 from Mycobacterium leprae. Based on its crystal structure, PHL forms a seven-bladed beta-propeller assembling into a homo-dimer with an inter-subunit disulfide bridge. Investigating complexes with different ligands revealed the existence of two sets of binding sites per monomer-the first type prefers l-fucose and its derivatives, whereas the second type can bind d-galactose. Based on the sequence analysis, PHL could contain up to twelve binding sites per monomer. PHL was shown to interact with all types of red blood cells and insect haemocytes. Interestingly, PHL inhibited the production of reactive oxygen species induced by zymosan A in human blood and antimicrobial activity both in human blood, serum and insect haemolymph. Concurrently, PHL increased the constitutive level of oxidants in the blood and induced melanisation in haemolymph. Our results suggest that PHL might play a crucial role in the interaction of P. asymbiotica with both human and insect hosts.

Characterization of novel bangle lectin from Photorhabdus asymbiotica with dual sugar-binding specificity and its effect on host immunity.,Jancarikova G, Houser J, Dobes P, Demo G, Hyrsl P, Wimmerova M PLoS Pathog. 2017 Aug 14;13(8):e1006564. doi: 10.1371/journal.ppat.1006564. PMID:28806750^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Jancarikova G, Houser J, Dobes P, Demo G, Hyrsl P, Wimmerova M. Characterization of novel bangle lectin from Photorhabdus asymbiotica with dual sugar-binding specificity and its effect on host immunity. PLoS Pathog. 2017 Aug 14;13(8):e1006564. doi: 10.1371/journal.ppat.1006564. PMID:28806750 doi:http://dx.doi.org/10.1371/journal.ppat.1006564

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5mxg"

@@ Line 1: / Line 1: @@
 ==Photorhabdus asymbiotica lectin (PHL) in complex with blood group H trisaccharide==
-<StructureSection load='5mxg' size='340' side='right' caption='[[5mxg]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+<StructureSection load='5mxg' size='340' side='right'caption='[[5mxg]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5mxg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Phoaa Phoaa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MXG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MXG FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5mxg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Photorhabdus_asymbiotica_subsp._asymbiotica_ATCC_43949 Photorhabdus asymbiotica subsp. asymbiotica ATCC 43949]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MXG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MXG FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PAU_00698 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=553480 PHOAA])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PRD_900036:H+type+2+antigen,+beta+anomer'>PRD_900036</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mxg OCA], [http://pdbe.org/5mxg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mxg RCSB], [http://www.ebi.ac.uk/pdbsum/5mxg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mxg ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mxg OCA], [https://pdbe.org/5mxg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mxg RCSB], [https://www.ebi.ac.uk/pdbsum/5mxg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mxg ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/C7BLE4_PHOAA C7BLE4_PHOAA]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Phoaa]]
+[[Category: Large Structures]]
-[[Category: Demo, G]]
+[[Category: Photorhabdus asymbiotica subsp. asymbiotica ATCC 43949]]
-[[Category: Houser, J]]
+[[Category: Demo G]]
-[[Category: Jancarikova, G]]
+[[Category: Houser J]]
-[[Category: Wimmerova, M]]
+[[Category: Jancarikova G]]
-[[Category: Bgh trisaccharide]]
+[[Category: Wimmerova M]]
-[[Category: Lectin]]
-[[Category: Seven-bladed beta-propeller]]
-[[Category: Sugar binding protein]]

5mxg

From Proteopedia

Current revision

Photorhabdus asymbiotica lectin (PHL) in complex with blood group H trisaccharide

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox