1k4j

From Proteopedia

(Difference between revisions)

Revision as of 07:40, 25 November 2020

Crystal Structure of the Acyl-homoserinelactone Synthase EsaI Complexed with Rhenate

Structural highlights

1k4j is a 1 chain structure with sequence from "aplanobacter_stawarti"_(smith_1898)_mcculloch_1918 "aplanobacter stawarti" (smith 1898) mcculloch 1918. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1kzf
Gene:	esaI/esaR cluster ("Aplanobacter stawarti" (Smith 1898) McCulloch 1918)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ESAI_PANSE] Required for the synthesis of OHHL (N-(3-oxohexanoyl)-L-homoserine lactone), an autoinducer molecule which binds to EsaR. OHHL is necessary for biosynthesis of EPS virulence factor (extracellular heteropolysaccharide) which plays a role in the development of Stewart's wilt on sweet corn.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Synthesis and detection of acyl-homoserine lactones (AHLs) enables many gram-negative bacteria to engage in quorum sensing, an intercellular signaling mechanism that activates differentiation to virulent and biofilm lifestyles. The AHL synthases catalyze acylation of S-adenosyl-L-methionine by acyl-acyl carrier protein and lactonization of the methionine moiety to give AHLs. The crystal structure of the AHL synthase, EsaI, determined at 1.8 A resolution, reveals a remarkable structural similarity to the N-acetyltransferases and defines a common phosphopantetheine binding fold as the catalytic core. Critical residues responsible for catalysis and acyl chain specificity have been identified from a modeled substrate complex and verified through functional analysis in vivo. A mechanism for the N-acylation of S-adenosyl-L-methionine by 3-oxo-hexanoyl-acyl carrier protein is proposed.

Structural basis and specificity of acyl-homoserine lactone signal production in bacterial quorum sensing.,Watson WT, Minogue TD, Val DL, von Bodman SB, Churchill ME Mol Cell. 2002 Mar;9(3):685-94. PMID:11931774^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Watson WT, Minogue TD, Val DL, von Bodman SB, Churchill ME. Structural basis and specificity of acyl-homoserine lactone signal production in bacterial quorum sensing. Mol Cell. 2002 Mar;9(3):685-94. PMID:11931774

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1k4j"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of the Acyl-homoserinelactone Synthase EsaI Complexed with Rhenate==
-<StructureSection load='1k4j' size='340' side='right' caption='[[1k4j]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+<StructureSection load='1k4j' size='340' side='right'caption='[[1k4j]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1k4j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"aplanobacter_stawarti"_(smith_1898)_mcculloch_1918 "aplanobacter stawarti" (smith 1898) mcculloch 1918]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K4J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K4J FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1k4j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"aplanobacter_stawarti"_(smith_1898)_mcculloch_1918 "aplanobacter stawarti" (smith 1898) mcculloch 1918]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K4J OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1K4J FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=REO:PERRHENATE'>REO</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=REO:PERRHENATE'>REO</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1kzf|1kzf]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1kzf|1kzf]]</div></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">esaI/esaR cluster ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=66271 "Aplanobacter stawarti" (Smith 1898) McCulloch 1918])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k4j OCA], [http://pdbe.org/1k4j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1k4j RCSB], [http://www.ebi.ac.uk/pdbsum/1k4j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1k4j ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1k4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k4j OCA], [http://pdbe.org/1k4j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1k4j RCSB], [http://www.ebi.ac.uk/pdbsum/1k4j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1k4j ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 34: / Line 34: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Bodman, S Beck von]]
 [[Category: Churchill, M E.A]]

1k4j

From Proteopedia

Revision as of 07:40, 25 November 2020

Crystal Structure of the Acyl-homoserinelactone Synthase EsaI Complexed with Rhenate

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox