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1l5a

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Revision as of 07:54, 25 November 2020

Crystal Structure of VibH, an NRPS Condensation Enzyme

Structural highlights

1l5a is a 3 chain structure with sequence from "bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	vibH ("Bacillo virgola del Koch" Trevisan 1884)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Nonribosomal peptide synthetases (NRPSs) are large, multidomain enzymes that biosynthesize medically important natural products. We report the crystal structure of the free-standing NRPS condensation (C) domain VibH, which catalyzes amide bond formation in the synthesis of vibriobactin, a Vibrio cholerae siderophore. Despite low sequence identity, NRPS condensation enzymes are structurally related to chloramphenicol acetyltransferase (CAT) and dihydrolipoamide acyltransferases. However, although the latter enzymes are homotrimers, VibH is a monomeric pseudodimer. The VibH structure is representative of both NRPS condensation and epimerization domains, as well as the condensation-variant cyclization domains, which are all expected to be monomers. Surprisingly, despite favorable positioning in the active site, a universally conserved histidine important in CAT and in other C domains is not critical for general base catalysis in VibH.

The structure of VibH represents nonribosomal peptide synthetase condensation, cyclization and epimerization domains.,Keating TA, Marshall CG, Walsh CT, Keating AE Nat Struct Biol. 2002 Jul;9(7):522-6. PMID:12055621^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Keating TA, Marshall CG, Walsh CT, Keating AE. The structure of VibH represents nonribosomal peptide synthetase condensation, cyclization and epimerization domains. Nat Struct Biol. 2002 Jul;9(7):522-6. PMID:12055621 doi:10.1038/nsb810

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1l5a"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of VibH, an NRPS Condensation Enzyme==
-<StructureSection load='1l5a' size='340' side='right' caption='[[1l5a]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
+<StructureSection load='1l5a' size='340' side='right'caption='[[1l5a]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1l5a]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1L5A FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1l5a]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1L5A FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">vibH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1l5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l5a OCA], [http://pdbe.org/1l5a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1l5a RCSB], [http://www.ebi.ac.uk/pdbsum/1l5a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1l5a ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1l5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l5a OCA], [http://pdbe.org/1l5a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1l5a RCSB], [http://www.ebi.ac.uk/pdbsum/1l5a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1l5a ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 31: / Line 31: @@
 </StructureSection>
 [[Category: Bacillo virgola del koch trevisan 1884]]
+[[Category: Large Structures]]
 [[Category: Keating, A E]]
 [[Category: Keating, T A]]

1l5a

From Proteopedia

Revision as of 07:54, 25 November 2020

Crystal Structure of VibH, an NRPS Condensation Enzyme

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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