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1q7f

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Revision as of 07:45, 9 December 2020

Brain Tumor NHL domain

Structural highlights

1q7f is a 2 chain structure with sequence from Drome. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	brain tumor (DROME)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[BRAT_DROME] Plays a central role in translation repression of hb mRNA by being recruited by nos and pum to the Nanos Response Element (NRE), a 16 bp sequence in the hb mRNA 3'-UTR. Probably recruited by other proteins to repress translation of other mRNAs in other tissues. Involved in the regulation of ribosomal RNA synthesis and cell growth. Participates in abdominal segmentation and imaginal disk development. Involved in brain tumor suppression, by acting as a growth suppressor in the larval brain.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The Brain Tumor (Brat) protein is recruited to the 3' untranslated region (UTR) of hunchback mRNA to regulate its translation. Recruitment is mediated by interactions between the Pumilio RNA-binding Puf repeats and the NHL domain of Brat, a conserved structural motif present in a large family of growth regulators. In this report, we describe the crystal structure of the Brat NHL domain and present a model of the Pumilio-Brat complex derived from in silico docking experiments and supported by mutational analysis of the protein-protein interface. A key feature of the model is recognition of the outer, convex surface of the Pumilio Puf domain by the top, electropositive face of the six-bladed Brat beta-propeller. In particular, an extended loop in Puf repeat 8 fits in the entrance to the central channel of the Brat beta-propeller. Together, these interactions are likely to be prototypic of the recruitment strategies of other NHL-containing proteins in development.

Model of the brain tumor-Pumilio translation repressor complex.,Edwards TA, Wilkinson BD, Wharton RP, Aggarwal AK Genes Dev. 2003 Oct 15;17(20):2508-13. PMID:14561773^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sonoda J, Wharton RP. Drosophila Brain Tumor is a translational repressor. Genes Dev. 2001 Mar 15;15(6):762-73. PMID:11274060 doi:http://dx.doi.org/10.1101/gad.870801
↑ Frank DJ, Edgar BA, Roth MB. The Drosophila melanogaster gene brain tumor negatively regulates cell growth and ribosomal RNA synthesis. Development. 2002 Jan;129(2):399-407. PMID:11807032
↑ Edwards TA, Wilkinson BD, Wharton RP, Aggarwal AK. Model of the brain tumor-Pumilio translation repressor complex. Genes Dev. 2003 Oct 15;17(20):2508-13. PMID:14561773 doi:10.1101/gad.1119403

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1q7f"

@@ Line 1: / Line 1: @@
 ==Brain Tumor NHL domain==
-<StructureSection load='1q7f' size='340' side='right' caption='[[1q7f]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
+<StructureSection load='1q7f' size='340' side='right'caption='[[1q7f]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1q7f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q7F FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1q7f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q7F OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1Q7F FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">brain tumor ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q7f OCA], [http://pdbe.org/1q7f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q7f RCSB], [http://www.ebi.ac.uk/pdbsum/1q7f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1q7f ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1q7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q7f OCA], [http://pdbe.org/1q7f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q7f RCSB], [http://www.ebi.ac.uk/pdbsum/1q7f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1q7f ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 33: / Line 33: @@
 </StructureSection>
 [[Category: Drome]]
+[[Category: Large Structures]]
 [[Category: Aggarwal, A K]]
 [[Category: Edwards, T A]]

1q7f

From Proteopedia

Revision as of 07:45, 9 December 2020

Brain Tumor NHL domain

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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