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2e4i

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[[Image:2e4i.gif|left|200px]]
[[Image:2e4i.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2e4i |SIZE=350|CAPTION= <scene name='initialview01'>2e4i</scene>
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The line below this paragraph, containing "STRUCTURE_2e4i", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=BGM:8-BROMO-2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>BGM</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_2e4i| PDB=2e4i | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2e4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e4i OCA], [http://www.ebi.ac.uk/pdbsum/2e4i PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2e4i RCSB]</span>
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}}
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'''Human Telomeric DNA mixed-parallel/antiparallel quadruplex under Physiological Ionic Conditions Stabilized by Proper Incorporation of 8-Bromoguanosines'''
'''Human Telomeric DNA mixed-parallel/antiparallel quadruplex under Physiological Ionic Conditions Stabilized by Proper Incorporation of 8-Bromoguanosines'''
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==About this Structure==
==About this Structure==
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2E4I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E4I OCA].
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2E4I is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E4I OCA].
==Reference==
==Reference==
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[[Category: Sugiyama, H.]]
[[Category: Sugiyama, H.]]
[[Category: Xu, Y.]]
[[Category: Xu, Y.]]
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[[Category: dna]]
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[[Category: Dna]]
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[[Category: mixed-parallel/antiparallel]]
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[[Category: Mixed-parallel/antiparallel]]
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[[Category: nmr]]
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[[Category: Nmr]]
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[[Category: quadruplex]]
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[[Category: Quadruplex]]
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[[Category: structure]]
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[[Category: Structure]]
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[[Category: telomere]]
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[[Category: Telomere]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 01:54:11 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:43:34 2008''
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Revision as of 22:54, 3 May 2008

Image:2e4i.gif

Template:STRUCTURE 2e4i

Human Telomeric DNA mixed-parallel/antiparallel quadruplex under Physiological Ionic Conditions Stabilized by Proper Incorporation of 8-Bromoguanosines


Overview

The structure of human telomeric DNA is controversial; it depends upon the sequence contexts and the methodologies used to determine it. The solution structure in the presence of K(+) is particularly interesting, but the structure is yet to be elucidated, due to possible conformational heterogeneity. Here, a unique strategy is applied to stabilize one such structure in a K(+) solution by substituting guanosines with 8-bromoguanosines at proper positions. The resulting spectra are cleaner and led to determination of the structure at a high atomic resolution. This demonstrates that the application of 8-bromoguanosine is a powerful tool to overcome the difficulty of nucleic acid structure determination arising from conformational heterogeneity. The obtained structure is a mixed-parallel/antiparallel quadruplex. The structure of telomeric DNA was recently reported in another study, in which stabilization was brought about by mutation and resultant additional interactions [Luu KN, Phan AT, Kuryavyi V, Lacroix L & Patel DJ (2006) Structure of the human telomere in K(+) solution: an intramolecular (3+1) G-quadruplex scaffold. J Am Chem Soc 128, 9963-9970]. The structure of the guanine tracts was similar between the two. However, a difference was seen for loops connecting guanine tracts, which may play a role in the higher order arrangement of telomeres. Our structure can be utilized to design a small molecule which stabilizes the quadruplex. This type of molecule is supposed to inhibit a telomerase and thus is expected to be a candidate anticancer drug.

About this Structure

2E4I is a Single protein structure. Full crystallographic information is available from OCA.

Reference

Structure of a human telomeric DNA sequence stabilized by 8-bromoguanosine substitutions, as determined by NMR in a K+ solution., Matsugami A, Xu Y, Noguchi Y, Sugiyama H, Katahira M, FEBS J. 2007 Jul;274(14):3545-56. Epub 2007 Jun 11. PMID:17561958 Page seeded by OCA on Sun May 4 01:54:11 2008

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