1mi7

From Proteopedia

(Difference between revisions)

Revision as of 08:37, 2 December 2020

Crystal Structure of Domain Swapped trp Aporepressor in 30%(v/v) Isopropanol

Structural highlights

1mi7 is a 1 chain structure with sequence from "bacillus_coli"_migula_1895 "bacillus coli" migula 1895. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1wrp, 2wrp, 3wrp, 1tro, 1trr
Gene:	trpR ("Bacillus coli" Migula 1895)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TRPR_ECOLI] This protein is an aporepressor. When complexed with L-tryptophan it binds the operator region of the trp operon (5'-ACTAGT-'3') and prevents the initiation of transcription. The complex also regulates trp repressor biosynthesis by binding to its regulatory region.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The E. coli trp repressor (trpR) homodimer recognizes its palindromic DNA binding site through a pair of flexible helix-turn-helix (HTH) motifs displayed on an intertwined helical core. Flexible N-terminal arms mediate association between dimers bound to tandem DNA sites. The 2.5 A X-ray structure of trpR crystallized in 30% (v/v) isopropanol reveals a substantial conformational rearrangement of HTH motifs and N-terminal arms, with the protein appearing in the unusual form of an ordered 3D domain-swapped supramolecular array. Small angle X-ray scattering measurements show that the self-association properties of trpR in solution are fundamentally altered by isopropanol.

E. coli trp repressor forms a domain-swapped array in aqueous alcohol.,Lawson CL, Benoff B, Berger T, Berman HM, Carey J Structure. 2004 Jun;12(6):1099-108. PMID:15274929^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lawson CL, Benoff B, Berger T, Berman HM, Carey J. E. coli trp repressor forms a domain-swapped array in aqueous alcohol. Structure. 2004 Jun;12(6):1099-108. PMID:15274929 doi:10.1016/j.str.2004.03.019

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1mi7"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of Domain Swapped trp Aporepressor in 30%(v/v) Isopropanol==
-<StructureSection load='1mi7' size='340' side='right' caption='[[1mi7]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+<StructureSection load='1mi7' size='340' side='right'caption='[[1mi7]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1mi7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MI7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1mi7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1MI7 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1wrp|1wrp]], [[2wrp|2wrp]], [[3wrp|3wrp]], [[1tro|1tro]], [[1trr|1trr]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1wrp|1wrp]], [[2wrp|2wrp]], [[3wrp|3wrp]], [[1tro|1tro]], [[1trr|1trr]]</div></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">trpR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mi7 OCA], [http://pdbe.org/1mi7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mi7 RCSB], [http://www.ebi.ac.uk/pdbsum/1mi7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mi7 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mi7 OCA], [http://pdbe.org/1mi7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mi7 RCSB], [http://www.ebi.ac.uk/pdbsum/1mi7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mi7 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 35: / Line 35: @@
 </StructureSection>
 [[Category: Bacillus coli migula 1895]]
+[[Category: Large Structures]]
 [[Category: Benoff, B]]
 [[Category: Berger, T]]

1mi7

From Proteopedia

Revision as of 08:37, 2 December 2020

Crystal Structure of Domain Swapped trp Aporepressor in 30%(v/v) Isopropanol

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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