2eb1

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2eb1.jpg|left|200px]]
[[Image:2eb1.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2eb1 |SIZE=350|CAPTION= <scene name='initialview01'>2eb1</scene>, resolution 2.00&Aring;
+
The line below this paragraph, containing "STRUCTURE_2eb1", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2eb1| PDB=2eb1 | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eb1 OCA], [http://www.ebi.ac.uk/pdbsum/2eb1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2eb1 RCSB]</span>
+
-
}}
+
'''Crystal Structure of the C-Terminal RNase III Domain of Human Dicer'''
'''Crystal Structure of the C-Terminal RNase III Domain of Human Dicer'''
Line 32: Line 29:
[[Category: Tanokura, M.]]
[[Category: Tanokura, M.]]
[[Category: Zenno, S.]]
[[Category: Zenno, S.]]
-
[[Category: endonuclease]]
+
[[Category: Endonuclease]]
-
[[Category: hydrolase]]
+
[[Category: Hydrolase]]
-
[[Category: nuclease]]
+
[[Category: Nuclease]]
-
[[Category: rna-binding]]
+
[[Category: Rna-binding]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 02:15:31 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:46:19 2008''
+

Revision as of 23:15, 3 May 2008

Image:2eb1.jpg

Template:STRUCTURE 2eb1

Crystal Structure of the C-Terminal RNase III Domain of Human Dicer


Overview

Human Dicer contains two RNase III domains (RNase IIIa and RNase IIIb) that are responsible for the production of short interfering RNAs and microRNAs. These small RNAs induce gene silencing known as RNA interference. Here, we report the crystal structure of the C-terminal RNase III domain (RNase IIIb) of human Dicer at 2.0 A resolution. The structure revealed that the RNase IIIb domain can form a tightly associated homodimer, which is similar to the dimers of the bacterial RNase III domains and the two RNase III domains of Giardia Dicer. Biochemical analysis showed that the RNase IIIb homodimer can cleave double-stranded RNAs (dsRNAs), and generate short dsRNAs with 2 nt 3' overhang, which is characteristic of RNase III products. The RNase IIIb domain contained two magnesium ions per monomer around the active site. The distance between two Mg-1 ions is approximately 20.6 A, almost identical with those observed in bacterial RNase III enzymes and Giardia Dicer, while the locations of two Mg-2 ions were not conserved at all. We presume that Mg-1 ions act as catalysts for dsRNA cleavage, while Mg-2 ions are involved in RNA binding.

About this Structure

2EB1 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Homodimeric structure and double-stranded RNA cleavage activity of the C-terminal RNase III domain of human dicer., Takeshita D, Zenno S, Lee WC, Nagata K, Saigo K, Tanokura M, J Mol Biol. 2007 Nov 16;374(1):106-20. Epub 2007 Sep 8. PMID:17920623 Page seeded by OCA on Sun May 4 02:15:31 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2eb1"
Personal tools