Structural highlights
Function
[KCND2_RAT] Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
We have developed a semiempirical algorithm called Family Values (FamVal), which identifies residues that encode functional specificity in a protein sequence. Given a multiple sequence alignment (MSA) grouped into functionally distinct subfamilies, FamVal calculates a specificity score for each subfamily at every amino acid position of an MSA. This algorithm was used to predict specificity-encoding positions within the tetramerization assembly (T1) domain of voltage-gated potassium (Kv) channel subfamilies Kv3 and Kv4. The importance of one such position (Arg to Ala at MSA position 93) was confirmed by in vitro pull-down assays. The structural basis of this assembly discrimination was elucidated by determining the crystal structure of the Kv4 T1 domain and comparing it to the Kv3 T1 domain.
Determining the basis of channel-tetramerization specificity by x-ray crystallography and a sequence-comparison algorithm: Family Values (FamVal).,Nanao MH, Zhou W, Pfaffinger PJ, Choe S Proc Natl Acad Sci U S A. 2003 Jul 22;100(15):8670-5. Epub 2003 Jun 30. PMID:12835418[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Baldwin TJ, Tsaur ML, Lopez GA, Jan YN, Jan LY. Characterization of a mammalian cDNA for an inactivating voltage-sensitive K+ channel. Neuron. 1991 Sep;7(3):471-83. PMID:1840649
- ↑ Nanao MH, Zhou W, Pfaffinger PJ, Choe S. Determining the basis of channel-tetramerization specificity by x-ray crystallography and a sequence-comparison algorithm: Family Values (FamVal). Proc Natl Acad Sci U S A. 2003 Jul 22;100(15):8670-5. Epub 2003 Jun 30. PMID:12835418 doi:10.1073/pnas.1432840100
