2eft
From Proteopedia
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'''Methanethiol-CYS 112 inhibition complex of E. coli ketoacyl synthase III (FABH) and Coenzyme A (high concentration (1.7mM) soak)''' | '''Methanethiol-CYS 112 inhibition complex of E. coli ketoacyl synthase III (FABH) and Coenzyme A (high concentration (1.7mM) soak)''' | ||
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[[Category: Scarsdale, N.]] | [[Category: Scarsdale, N.]] | ||
[[Category: Wright, H T.]] | [[Category: Wright, H T.]] | ||
| - | [[Category: | + | [[Category: Alkyl-coa-disulfide]] |
| - | [[Category: | + | [[Category: E. coli]] |
| - | [[Category: | + | [[Category: Fatty acid biosynthesis]] |
| - | [[Category: | + | [[Category: Half sites occupancy]] |
| - | [[Category: | + | [[Category: Mechanism-based inhibitor]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 02:29:04 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 23:29, 3 May 2008
Methanethiol-CYS 112 inhibition complex of E. coli ketoacyl synthase III (FABH) and Coenzyme A (high concentration (1.7mM) soak)
Overview
The first step of the reaction catalyzed by the homodimeric FabH from a dissociated fatty acid synthase is acyl transfer from acyl-CoA to an active site cysteine. We report that C1 to C10 alkyl-CoA disulfides irreversibly inhibit Escherichia coli FabH (ecFabH) and Mycobacterium tuberculosis FabH with relative efficiencies that reflect these enzymes' differential acyl-group specificity. Crystallographic and kinetic studies with MeSSCoA show rapid inhibition of one monomer of ecFabH through formation of a methyl disulfide conjugate with this cysteine. Reaction of the second subunit with either MeSSCoA or acetyl-CoA is much slower. In the presence of malonyl-ACP, the acylation rate of the second subunit is restored to that of the native ecFabH. These observations suggest a catalytic model in which a structurally disordered apo-ecFabH dimer orders on binding either the first substrate, acetyl-CoA, or the inhibitor MeSSCoA, and is restored to a disordered state on binding of malonyl-ACP.
About this Structure
2EFT is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Alkyl-CoA disulfides as inhibitors and mechanistic probes for FabH enzymes., Alhamadsheh MM, Musayev F, Komissarov AA, Sachdeva S, Wright HT, Scarsdale N, Florova G, Reynolds KA, Chem Biol. 2007 May;14(5):513-24. PMID:17524982 Page seeded by OCA on Sun May 4 02:29:04 2008
Categories: Beta-ketoacyl-acyl-carrier-protein synthase I | Escherichia coli | Single protein | Alhamadsheh, M M. | Florova, G. | Komissarov, A A. | Musayev, F. | Reynolds, K A. | Sachdeva, S. | Scarsdale, N. | Wright, H T. | Alkyl-coa-disulfide | E. coli | Fatty acid biosynthesis | Half sites occupancy | Mechanism-based inhibitor
