6cca
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of DszA carbon methyltransferase== | |
| + | <StructureSection load='6cca' size='340' side='right' caption='[[6cca]], [[Resolution|resolution]] 1.75Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6cca]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CCA FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cca OCA], [http://pdbe.org/6cca PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cca RCSB], [http://www.ebi.ac.uk/pdbsum/6cca PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cca ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The methyl substituents in products of trans-acyltransferase assembly lines are usually incorporated by S-adenosyl-methionine (SAM)-dependent methyltransferase (MT) domains. The gem-dimethyl moieties within the polyketide disorazol are installed through the iterative action of an MT in the third module of its assembly line. The 1.75-A-resolution crystal structure of this MT helps elucidate how it catalyzes the addition of two methyl groups. Activity assays of point mutants on beta-ketoacyl chains linked to an acyl carrier protein and N-acetylcysteamine provide additional insights into the roles of active site residues. The replacement of an alanine with a phenylalanine at an apparent gatekeeping position resulted in more monomethylation than dimethylation. MTs may form an interface with ketoreductases (KRs) and even mediate the docking of trans-acyltransferase assembly line polypeptides through this association. | ||
| - | + | Structural and Functional Studies of a gem-Dimethylating Methyltransferase from a trans-Acyltransferase Assembly Line.,Meinke JL, Mehaffey MR, Wagner DT, Sun N, Zhang Z, Brodbelt JS, Keatinge-Clay AT ACS Chem Biol. 2018 Nov 9. doi: 10.1021/acschembio.8b00733. PMID:30371052<ref>PMID:30371052</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6cca" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Keatinge-Clay, A T]] | ||
| + | [[Category: Meinke, J L]] | ||
| + | [[Category: Disorazol]] | ||
| + | [[Category: Gem-dimethyl]] | ||
| + | [[Category: Methyltransferase]] | ||
| + | [[Category: Polyketide assembly line]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 06:33, 5 December 2018
Crystal structure of DszA carbon methyltransferase
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