5xaq
From Proteopedia
(Difference between revisions)
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<StructureSection load='5xaq' size='340' side='right' caption='[[5xaq]], [[Resolution|resolution]] 1.86Å' scene=''> | <StructureSection load='5xaq' size='340' side='right' caption='[[5xaq]], [[Resolution|resolution]] 1.86Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5xaq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XAQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XAQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5xaq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XAQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XAQ FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xaq OCA], [http://pdbe.org/5xaq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xaq RCSB], [http://www.ebi.ac.uk/pdbsum/5xaq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xaq ProSAT]</span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Dtd2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xaq OCA], [http://pdbe.org/5xaq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xaq RCSB], [http://www.ebi.ac.uk/pdbsum/5xaq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xaq ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/DTD2_MOUSE DTD2_MOUSE]] May hydrolyze D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr). Could be a defense mechanism against a harmful effect of D-tyrosine (Potential). | [[http://www.uniprot.org/uniprot/DTD2_MOUSE DTD2_MOUSE]] May hydrolyze D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr). Could be a defense mechanism against a harmful effect of D-tyrosine (Potential). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | D-aminoacyl-tRNA deacylase (DTD), a bacterial/eukaryotic trans-editing factor, removes D-amino acids mischarged on tRNAs and achiral glycine mischarged on tRNA(Ala). An invariant cross-subunit Gly-cisPro motif forms the mechanistic basis of L-amino acid rejection from the catalytic site. Here, we present the identification of a DTD variant, named ATD (Animalia-specific tRNA deacylase), that harbors a Gly-transPro motif. The cis-to-trans switch causes a "gain of function" through L-chiral selectivity in ATD resulting in the clearing of L-alanine mischarged on tRNA(Thr)(G4*U69) by eukaryotic AlaRS. The proofreading activity of ATD is conserved across diverse classes of phylum Chordata. Animalia genomes enriched in tRNA(Thr)(G4*U69) genes are in strict association with the presence of ATD, underlining the mandatory requirement of a dedicated factor to proofread tRNA misaminoacylation. The study highlights the emergence of ATD during genome expansion as a key event associated with the evolution of Animalia. | ||
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| + | A chiral selectivity relaxed paralog of DTD for proofreading tRNA mischarging in Animalia.,Kuncha SK, Mazeed M, Singh R, Kattula B, Routh SB, Sankaranarayanan R Nat Commun. 2018 Feb 6;9(1):511. doi: 10.1038/s41467-017-02204-w. PMID:29410408<ref>PMID:29410408</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5xaq" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Lk3 transgenic mice]] | ||
[[Category: Kattula, B]] | [[Category: Kattula, B]] | ||
[[Category: Kuncha, K S]] | [[Category: Kuncha, K S]] | ||
Revision as of 08:23, 22 February 2018
Crystal structure of Animalia-specific tRNA deacylase from Mus musculus
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