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2ek8

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[[Image:2ek8.jpg|left|200px]]
[[Image:2ek8.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2ek8 |SIZE=350|CAPTION= <scene name='initialview01'>2ek8</scene>, resolution 1.80&Aring;
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The line below this paragraph, containing "STRUCTURE_2ek8", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Zn+Binding+Site+For+Residue+A+1001'>AC1</scene>, <scene name='pdbsite=AC2:Zn+Binding+Site+For+Residue+A+1002'>AC2</scene>, <scene name='pdbsite=AC3:Zn+Binding+Site+For+Residue+A+1003'>AC3</scene>, <scene name='pdbsite=AC4:Zn+Binding+Site+For+Residue+A+1004'>AC4</scene>, <scene name='pdbsite=AC5:Zn+Binding+Site+For+Residue+A+1005'>AC5</scene>, <scene name='pdbsite=AC6:Zn+Binding+Site+For+Residue+A+1006'>AC6</scene> and <scene name='pdbsite=AC7:Ipa+Binding+Site+For+Residue+A+2001'>AC7</scene>
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|LIGAND= <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam04389 Peptidase_M28], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd02133 PA_C5a_like]</span>
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{{STRUCTURE_2ek8| PDB=2ek8 | SCENE= }}
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|RELATEDENTRY=[[2ek9|2EK9]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ek8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ek8 OCA], [http://www.ebi.ac.uk/pdbsum/2ek8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ek8 RCSB]</span>
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'''Aminopeptidase from Aneurinibacillus sp. strain AM-1'''
'''Aminopeptidase from Aneurinibacillus sp. strain AM-1'''
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[[Category: Nakano, H.]]
[[Category: Nakano, H.]]
[[Category: Watanabe, K.]]
[[Category: Watanabe, K.]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: metalloproteinase]]
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[[Category: Metalloproteinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 02:40:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:50:27 2008''
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Revision as of 23:40, 3 May 2008

Image:2ek8.jpg

Template:STRUCTURE 2ek8

Aminopeptidase from Aneurinibacillus sp. strain AM-1


Overview

To elucidate the structure and molecular mechanism of a characteristic proline-specific aminopeptidase produced by the thermophile Aneurinibacillus sp. strain AM-1, its gene was cloned and the recombinant protein was overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 1.8 A resolution from the recombinant aminopeptidase crystal. The crystals belong to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 93.62, b = 68.20, c = 76.84 A. A complete data set was also obtained from crystals of SeMet-substituted aminopeptidase. Data in the resolution range 20-2.1 A from the MAD data set from the SeMet-substituted crystal were used for phase determination.

About this Structure

2EK8 is a Single protein structure of sequence from Bacteria. Full crystallographic information is available from OCA.

Reference

Overexpression, purification, crystallization and preliminary X-ray crystallographic studies of a proline-specific aminopeptidase from Aneurinibacillus sp. strain AM-1., Akioka M, Nakano H, Horikiri A, Tsujimoto Y, Matsui H, Shimizu T, Nakatsu T, Kato H, Watanabe K, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Dec 1;62(Pt, 12):1266-8. Epub 2006 Nov 30. PMID:17142913 Page seeded by OCA on Sun May 4 02:40:23 2008

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