1p51

From Proteopedia

(Difference between revisions)

Revision as of 07:22, 9 December 2020

Anabaena HU-DNA cocrystal structure (AHU6)

Structural highlights

1p51 is a 8 chain structure with sequence from Anabaena sp.. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1b8z, 1ihf, 1p71, 1p78
Gene:	HUP OR HANA OR ASR3935 (Anabaena sp.)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[DBH_NOSS1] Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions. It is essential for heterocyst differentiation.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

HU and IHF are members of a family of prokaryotic proteins that interact with the DNA minor groove in a sequence-specific (IHF) or non-specific (HU) manner to induce and/or stabilize DNA bending. HU plays architectural roles in replication initiation, transcription regulation and site-specific recombination, and is associated with bacterial nucleoids. Cocrystal structures of Anabaena HU bound to DNA (1P71, 1P78, 1P51) reveal that while underlying proline intercalation and asymmetric charge neutralization mechanisms of DNA bending are similar for IHF and HU, HU stabilizes different DNA bend angles ( approximately 105-140 degrees ). The two bend angles within a single HU complex are not coplanar, and the resulting dihedral angle is consistent with negative supercoiling. Comparison of HU-DNA and IHF-DNA structures suggests that sharper bending is correlated with longer DNA binding sites and smaller dihedral angles. An HU-induced bend may be better modeled as a hinge, not a rigid bend. The ability to induce or stabilize varying bend angles is consistent with HU's role as an architectural cofactor in many different systems that may require differing geometries.

Flexible DNA bending in HU-DNA cocrystal structures.,Swinger KK, Lemberg KM, Zhang Y, Rice PA EMBO J. 2003 Jul 15;22(14):3749-60. PMID:12853489^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Swinger KK, Lemberg KM, Zhang Y, Rice PA. Flexible DNA bending in HU-DNA cocrystal structures. EMBO J. 2003 Jul 15;22(14):3749-60. PMID:12853489 doi:10.1093/emboj/cdg351

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1p51"

@@ Line 1: / Line 1: @@
 ==Anabaena HU-DNA cocrystal structure (AHU6)==
-<StructureSection load='1p51' size='340' side='right' caption='[[1p51]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+<StructureSection load='1p51' size='340' side='right'caption='[[1p51]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1p51]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P51 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1P51 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1p51]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P51 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1P51 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1b8z|1b8z]], [[1ihf|1ihf]], [[1p71|1p71]], [[1p78|1p78]]</td></tr>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1b8z|1b8z]], [[1ihf|1ihf]], [[1p71|1p71]], [[1p78|1p78]]</div></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HUP OR HANA OR ASR3935 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1167 Anabaena sp.])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p51 OCA], [http://pdbe.org/1p51 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1p51 RCSB], [http://www.ebi.ac.uk/pdbsum/1p51 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1p51 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1p51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p51 OCA], [http://pdbe.org/1p51 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1p51 RCSB], [http://www.ebi.ac.uk/pdbsum/1p51 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1p51 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 34: / Line 34: @@
 </StructureSection>
 [[Category: Anabaena sp]]
+[[Category: Large Structures]]
 [[Category: Lemberg, K M]]
 [[Category: Rice, P A]]

1p51

From Proteopedia

Revision as of 07:22, 9 December 2020

Anabaena HU-DNA cocrystal structure (AHU6)

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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