This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5uki
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Mn2+ and Zn2+ requirements for the lariat debranching enzyme, Dbr1== | ==Mn2+ and Zn2+ requirements for the lariat debranching enzyme, Dbr1== | ||
| - | <StructureSection load='5uki' size='340' side='right' caption='[[5uki]], [[Resolution|resolution]] 1.80Å' scene=''> | + | <StructureSection load='5uki' size='340' side='right' caption='[[5uki]], [[Resolution|resolution]] 1.80Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5uki]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UKI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UKI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5uki]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enthi Enthi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UKI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UKI FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EHI_062730 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5759 ENTHI])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uki OCA], [http://pdbe.org/5uki PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uki RCSB], [http://www.ebi.ac.uk/pdbsum/5uki PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uki ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uki OCA], [http://pdbe.org/5uki PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uki RCSB], [http://www.ebi.ac.uk/pdbsum/5uki PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uki ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The RNA lariat debranching enzyme, Dbr1, is a metallophosphoesterase that cleaves 2'-5' phosphodiester bonds within intronic lariats. Previous reports have indicated that Dbr1 enzymatic activity is supported by diverse metal ions including Ni(2+) , Mn(2+) , Mg(2+) , Fe(2+) , and Zn(2+) . While in initial structures of the Entamoeba histolytica Dbr1 only one of the two catalytic metal-binding sites were observed to be occupied (with a Mn(2+) ion), recent structures determined a Zn(2+) /Fe(2+) heterobinucleation. We solved a high-resolution X-ray crystal structure (1.8 A) of the E. histolytica Dbr1 and determined a Zn(2+) /Mn(2+) occupancy. ICP-AES corroborate this finding, and in vitro debranching assays with fluorescently labeled branched substrates confirm activity. | ||
| + | |||
| + | Crystal structure of the Entamoeba histolytica RNA lariat debranching enzyme EhDbr1 reveals a catalytic Zn(2+) /Mn(2+) heterobinucleation.,Ransey E, Paredes E, Dey SK, Das SR, Heroux A, Macbeth MR FEBS Lett. 2017 Jul;591(13):2003-2010. doi: 10.1002/1873-3468.12677. Epub 2017, Jun 14. PMID:28504306<ref>PMID:28504306</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5uki" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Enthi]] | ||
[[Category: Macbeth, M R]] | [[Category: Macbeth, M R]] | ||
[[Category: Ransey, L]] | [[Category: Ransey, L]] | ||
Revision as of 08:17, 6 March 2019
Mn2+ and Zn2+ requirements for the lariat debranching enzyme, Dbr1
| |||||||||||
Categories: Enthi | Macbeth, M R | Ransey, L | Debranching | Hydrolase | Metal ion | Rna
