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2erb
From Proteopedia
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'''AgamOBP1, and odorant binding protein from Anopheles gambiae complexed with PEG''' | '''AgamOBP1, and odorant binding protein from Anopheles gambiae complexed with PEG''' | ||
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[[Category: Wilson, D K.]] | [[Category: Wilson, D K.]] | ||
[[Category: Wogulis, M.]] | [[Category: Wogulis, M.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:01:47 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 00:01, 4 May 2008
AgamOBP1, and odorant binding protein from Anopheles gambiae complexed with PEG
Overview
The Anopheles gambiae mosquito is the main vector of malaria transmission in sub-Saharan Africa. We present here a 1.5A crystal structure of AgamOBP1, an odorant binding protein (OBP) from the A. gambiae mosquito. The protein crystallized as a dimer with a unique binding pocket consisting of a continuous tunnel running through both subunits of the dimer and occupied by a PEG molecule. We demonstrate that AgamOBP1 undergoes a pH dependent conformational change that is associated with reduced ligand binding. A predominance of acid-labile hydrogen bonds involving the C-terminal loop suggests a mechanism in which a drop in pH causes C-terminal loop to open, leaving the binding tunnel solvent exposed, thereby lowering binding affinity for ligand. Because proteins from two distantly related insects also undergo a pH dependent conformational change involving the C-terminus that is associated with reduced ligand affinity, our results suggest a common mechanism for OBP activity.
About this Structure
2ERB is a Single protein structure of sequence from Anopheles gambiae. Full crystallographic information is available from OCA.
Reference
The crystal structure of an odorant binding protein from Anopheles gambiae: evidence for a common ligand release mechanism., Wogulis M, Morgan T, Ishida Y, Leal WS, Wilson DK, Biochem Biophys Res Commun. 2006 Jan 6;339(1):157-64. Epub 2005 Nov 9. PMID:16300742 Page seeded by OCA on Sun May 4 03:01:47 2008
