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| | ==Structure of formate channel== | | ==Structure of formate channel== |
| - | <StructureSection load='3kcv' size='340' side='right' caption='[[3kcv]], [[Resolution|resolution]] 3.20Å' scene=''> | + | <StructureSection load='3kcv' size='340' side='right'caption='[[3kcv]], [[Resolution|resolution]] 3.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3kcv]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KCV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KCV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3kcv]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KCV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KCV FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3kcu|3kcu]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.198Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FOCA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kcv OCA], [https://pdbe.org/3kcv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kcv RCSB], [https://www.ebi.ac.uk/pdbsum/3kcv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kcv ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kcv OCA], [http://pdbe.org/3kcv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3kcv RCSB], [http://www.ebi.ac.uk/pdbsum/3kcv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3kcv ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/FOCA_ECO57 FOCA_ECO57]] Involved in the bidirectional transport of formate (By similarity). | + | [https://www.uniprot.org/uniprot/FOCA_ECO57 FOCA_ECO57] Involved in the bidirectional transport of formate (By similarity). |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Eco57]] | + | [[Category: Escherichia coli O157:H7]] |
| - | [[Category: Huang, Y]] | + | [[Category: Large Structures]] |
| - | [[Category: Shi, Y]] | + | [[Category: Huang Y]] |
| - | [[Category: Wang, J]] | + | [[Category: Shi Y]] |
| - | [[Category: Wang, Y]] | + | [[Category: Wang J]] |
| - | [[Category: Yan, N]] | + | [[Category: Wang Y]] |
| - | [[Category: Cell inner membrane]]
| + | [[Category: Yan N]] |
| - | [[Category: Cell membrane]]
| + | |
| - | [[Category: Channel]]
| + | |
| - | [[Category: Formate]]
| + | |
| - | [[Category: Membrane]]
| + | |
| - | [[Category: Tcdb id 2 a.44 1.1]]
| + | |
| - | [[Category: Transmembrane]]
| + | |
| - | [[Category: Transport]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| - | [[Category: Transporter]]
| + | |
| Structural highlights
Function
FOCA_ECO57 Involved in the bidirectional transport of formate (By similarity).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
FocA is a representative member of the formate-nitrite transporter family, which transports short-chain acids in bacteria, archaea, fungi, algae and parasites. The structure and transport mechanism of the formate-nitrite transporter family remain unknown. Here we report the crystal structure of Escherichia coli FocA at 2.25 A resolution. FocA forms a symmetric pentamer, with each protomer consisting of six transmembrane segments. Despite a lack of sequence homology, the overall structure of the FocA protomer closely resembles that of aquaporin and strongly argues that FocA is a channel, rather than a transporter. Structural analysis identifies potentially important channel residues, defines the channel path and reveals two constriction sites. Unlike aquaporin, FocA is impermeable to water but allows the passage of formate. A structural and biochemical investigation provides mechanistic insights into the channel activity of FocA.
Structure of the formate transporter FocA reveals a pentameric aquaporin-like channel.,Wang Y, Huang Y, Wang J, Cheng C, Huang W, Lu P, Xu YN, Wang P, Yan N, Shi Y Nature. 2009 Nov 26;462(7272):467-72. PMID:19940917[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wang Y, Huang Y, Wang J, Cheng C, Huang W, Lu P, Xu YN, Wang P, Yan N, Shi Y. Structure of the formate transporter FocA reveals a pentameric aquaporin-like channel. Nature. 2009 Nov 26;462(7272):467-72. PMID:19940917 doi:http://dx.doi.org/10.1038/nature08610
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