2ey4
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2ey4.gif|left|200px]] | [[Image:2ey4.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2ey4", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2ey4| PDB=2ey4 | SCENE= }} | |
| - | | | + | |
| - | | | + | |
| - | }} | + | |
'''Crystal Structure of a Cbf5-Nop10-Gar1 Complex''' | '''Crystal Structure of a Cbf5-Nop10-Gar1 Complex''' | ||
| Line 30: | Line 27: | ||
[[Category: Rashid, R.]] | [[Category: Rashid, R.]] | ||
[[Category: SECSG, Southeast Collaboratory for Structural Genomics.]] | [[Category: SECSG, Southeast Collaboratory for Structural Genomics.]] | ||
| - | [[Category: | + | [[Category: Protein structure initiative]] |
| - | [[Category: | + | [[Category: Psi]] |
| - | [[Category: | + | [[Category: Secsg]] |
| - | [[Category: | + | [[Category: Southeast collaboratory for structural genomic]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | [[Category: | + | [[Category: Trimeric complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:14:52 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 00:14, 4 May 2008
Crystal Structure of a Cbf5-Nop10-Gar1 Complex
Overview
H/ACA RNA-protein complexes, comprised of four proteins and an H/ACA guide RNA, modify ribosomal and small nuclear RNAs. The H/ACA proteins are also essential components of telomerase in mammals. Cbf5 is the H/ACA protein that catalyzes isomerization of uridine to pseudouridine in target RNAs. Mutations in human Cbf5 (dyskerin) lead to dyskeratosis congenita. Here, we describe the 2.1 A crystal structure of a specific complex of three archaeal H/ACA proteins, Cbf5, Nop10, and Gar1. Cbf5 displays structural properties that are unique among known pseudouridine synthases and are consistent with its distinct function in RNA-guided pseudouridylation. We also describe the previously unknown structures of both Nop10 and Gar1 and the structural basis for their essential roles in pseudouridylation. By using information from related structures, we have modeled the entire ribonucleoprotein complex including both guide and substrate RNAs. We have also identified a dyskeratosis congenita mutation cluster site within a modeled dyskerin structure.
About this Structure
2EY4 is a Protein complex structure of sequences from Pyrococcus furiosus and Pyrococcus furiosus dsm 3638. Full crystallographic information is available from OCA.
Reference
Crystal structure of a Cbf5-Nop10-Gar1 complex and implications in RNA-guided pseudouridylation and dyskeratosis congenita., Rashid R, Liang B, Baker DL, Youssef OA, He Y, Phipps K, Terns RM, Terns MP, Li H, Mol Cell. 2006 Jan 20;21(2):249-60. PMID:16427014 Page seeded by OCA on Sun May 4 03:14:52 2008
Categories: Protein complex | Pyrococcus furiosus | Pyrococcus furiosus dsm 3638 | Li, H. | Liang, B. | Rashid, R. | SECSG, Southeast Collaboratory for Structural Genomics. | Protein structure initiative | Psi | Secsg | Southeast collaboratory for structural genomic | Structural genomic | Trimeric complex
