2ez4

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[[Image:2ez4.gif|left|200px]]
[[Image:2ez4.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2ez4 |SIZE=350|CAPTION= <scene name='initialview01'>2ez4</scene>, resolution 2.03&Aring;
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The line below this paragraph, containing "STRUCTURE_2ez4", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyruvate_oxidase Pyruvate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.3.3 1.2.3.3] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= pox5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1590 Lactobacillus plantarum])
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-->
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|DOMAIN=
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{{STRUCTURE_2ez4| PDB=2ez4 | SCENE= }}
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|RELATEDENTRY=[[1pox|1POX]], [[1pow|1POW]], [[1y9d|1Y9D]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ez4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ez4 OCA], [http://www.ebi.ac.uk/pdbsum/2ez4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ez4 RCSB]</span>
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}}
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'''Pyruvate oxidase variant F479W'''
'''Pyruvate oxidase variant F479W'''
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[[Category: Tittmann, K.]]
[[Category: Tittmann, K.]]
[[Category: Wille, G.]]
[[Category: Wille, G.]]
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[[Category: tpp enzyme]]
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[[Category: Tpp enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:16:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:55:40 2008''
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Revision as of 00:16, 4 May 2008

Image:2ez4.gif

Template:STRUCTURE 2ez4

Pyruvate oxidase variant F479W


Overview

Enzymes that use the cofactor thiamin diphosphate (ThDP, 1), the biologically active form of vitamin B(1), are involved in numerous metabolic pathways in all organisms. Although a theory of the cofactor's underlying reaction mechanism has been established over the last five decades, the three-dimensional structures of most major reaction intermediates of ThDP enzymes have remained elusive. Here, we report the X-ray structures of key intermediates in the oxidative decarboxylation of pyruvate, a central reaction in carbon metabolism catalyzed by the ThDP- and flavin-dependent enzyme pyruvate oxidase (POX)3 from Lactobacillus plantarum. The structures of 2-lactyl-ThDP (LThDP, 2) and its stable phosphonate analog, of 2-hydroxyethyl-ThDP (HEThDP, 3) enamine and of 2-acetyl-ThDP (AcThDP, 4; all shown bound to the enzyme's active site) provide profound insights into the chemical mechanisms and the stereochemical course of thiamin catalysis. These snapshots also suggest a mechanism for a phosphate-linked acyl transfer coupled to electron transfer in a radical reaction of pyruvate oxidase.

About this Structure

2EZ4 is a Single protein structure of sequence from Lactobacillus plantarum. Full crystallographic information is available from OCA.

Reference

The catalytic cycle of a thiamin diphosphate enzyme examined by cryocrystallography., Wille G, Meyer D, Steinmetz A, Hinze E, Golbik R, Tittmann K, Nat Chem Biol. 2006 Jun;2(6):324-8. Epub 2006 May 7. PMID:16680160 Page seeded by OCA on Sun May 4 03:16:22 2008

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