2ez6

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[[Image:2ez6.gif|left|200px]]
[[Image:2ez6.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2ez6 |SIZE=350|CAPTION= <scene name='initialview01'>2ez6</scene>, resolution 2.05&Aring;
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The line below this paragraph, containing "STRUCTURE_2ez6", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= rnc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus])
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-->
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|DOMAIN=
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{{STRUCTURE_2ez6| PDB=2ez6 | SCENE= }}
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|RELATEDENTRY=[[1jfz|1JFZ]], [[1o0w|1O0W]], [[1rc7|1RC7]], [[1yz9|1YZ9]], [[1yyo|1YYO]], [[1yyw|1YYW]], [[1rc5|1RC5]], [[1yyk|1YYK]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ez6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ez6 OCA], [http://www.ebi.ac.uk/pdbsum/2ez6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ez6 RCSB]</span>
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}}
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'''Crystal structure of Aquifex aeolicus RNase III (D44N) complexed with product of double-stranded RNA processing'''
'''Crystal structure of Aquifex aeolicus RNase III (D44N) complexed with product of double-stranded RNA processing'''
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[[Category: Tropea, J E.]]
[[Category: Tropea, J E.]]
[[Category: Waugh, D S.]]
[[Category: Waugh, D S.]]
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[[Category: dsrna]]
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[[Category: Dsrna]]
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[[Category: ribonuclease iii]]
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[[Category: Ribonuclease iii]]
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[[Category: rna interference]]
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[[Category: Rna interference]]
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[[Category: rna processing]]
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[[Category: Rna processing]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:16:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:55:48 2008''
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Revision as of 00:16, 4 May 2008

Image:2ez6.gif

Template:STRUCTURE 2ez6

Crystal structure of Aquifex aeolicus RNase III (D44N) complexed with product of double-stranded RNA processing


Overview

Members of the ribonuclease III (RNase III) family are double-stranded RNA (dsRNA) specific endoribonucleases characterized by a signature motif in their active centers and a two-base 3' overhang in their products. While Dicer, which produces small interfering RNAs, is currently the focus of intense interest, the structurally simpler bacterial RNase III serves as a paradigm for the entire family. Here, we present the crystal structure of an RNase III-product complex, the first catalytic complex observed for the family. A 7 residue linker within the protein facilitates induced fit in protein-RNA recognition. A pattern of protein-RNA interactions, defined by four RNA binding motifs in RNase III and three protein-interacting boxes in dsRNA, is responsible for substrate specificity, while conserved amino acid residues and divalent cations are responsible for scissile-bond cleavage. The structure reveals a wealth of information about the mechanism of RNA hydrolysis that can be extrapolated to other RNase III family members.

About this Structure

2EZ6 is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.

Reference

Structural insight into the mechanism of double-stranded RNA processing by ribonuclease III., Gan J, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X, Cell. 2006 Jan 27;124(2):355-66. PMID:16439209 Page seeded by OCA on Sun May 4 03:16:31 2008

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