2f1e
From Proteopedia
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'''Solution structure of ApaG protein''' | '''Solution structure of ApaG protein''' | ||
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[[Category: Pertinhez, T A.]] | [[Category: Pertinhez, T A.]] | ||
[[Category: Spisni, A.]] | [[Category: Spisni, A.]] | ||
| - | [[Category: | + | [[Category: Apag protein]] |
| - | [[Category: | + | [[Category: Nmr]] |
| - | [[Category: | + | [[Category: Xanthomonas axonopodis pv citri]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:20:55 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 00:20, 4 May 2008
Solution structure of ApaG protein
Overview
ApaG proteins are found in a wide variety of bacterial genomes but their function is as yet unknown. Some eukaryotic proteins involved in protein-protein interactions, such as the human polymerase delta-interacting protein (PDIP38) and the F Box A (FBA) proteins, contain ApaG homology domains. We have used NMR to determine the solution structure of ApaG protein from the plant pathogen Xanthomonas axonopodis pv. citri (ApaG(Xac)) with the aim to shed some light on its molecular function. ApaG(Xac) is characterized by seven antiparallel beta strands forming two beta sheets, one containing three strands (ABE) and the other four strands (GFCC'). Relaxation measurements indicate that the protein has a quite rigid structure. In spite of the presence of a putative GXGXXG pyrophosphate binding motif ApaG(Xac) does not bind ATP or GTP, in vitro. On the other hand, ApaG(Xac) adopts a fibronectin type III (Fn3) fold, which is consistent with the hypothesis that it is involved in mediating protein-protein interactions. The fact that the proteins of ApaG family do not display significant sequence similarity with the Fn3 domains found in other eukaryotic or bacterial proteins suggests that Fn3 domain may have arisen earlier in evolution than previously estimated.
About this Structure
2F1E is a Single protein structure of sequence from Xanthomonas axonopodis pv. citri. Full crystallographic information is available from OCA.
Reference
Solution structure of ApaG from Xanthomonas axonopodis pv. citri reveals a fibronectin-3 fold., Cicero DO, Contessa GM, Pertinhez TA, Gallo M, Katsuyama AM, Paci M, Farah CS, Spisni A, Proteins. 2007 May 1;67(2):490-500. PMID:17256769 Page seeded by OCA on Sun May 4 03:20:55 2008
