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5uza
From Proteopedia
(Difference between revisions)
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uza OCA], [http://pdbe.org/5uza PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uza RCSB], [http://www.ebi.ac.uk/pdbsum/5uza PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uza ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uza OCA], [http://pdbe.org/5uza PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uza RCSB], [http://www.ebi.ac.uk/pdbsum/5uza PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uza ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Site-specific incorporation of labeled nucleotides is an extremely useful synthetic tool for many structural studies (e.g., NMR, electron paramagnetic resonance (EPR), fluorescence resonance energy transfer (FRET), and X-ray crystallography) of RNA. However, specific-position-labeled RNAs >60 nt are not commercially available on a milligram scale. Position-selective labeling of RNA (PLOR) has been applied to prepare large RNAs labeled at desired positions, and all the required reagents are commercially available. Here, we present a step-by-step protocol for the solid-liquid hybrid phase method PLOR to synthesize 71-nt RNA samples with three different modification applications, containing (i) a (13)C(15)N-labeled segment; (ii) discrete residues modified with Cy3, Cy5, or biotin; or (iii) two iodo-U residues. The flexible procedure enables a wide range of downstream biophysical analyses using precisely localized functionalized nucleotides. All three RNAs were obtained in <2 d, excluding time for preparing reagents and optimizing experimental conditions. With optimization, the protocol can be applied to other RNAs with various labeling schemes, such as ligation of segmentally labeled fragments. | ||
| + | |||
| + | Incorporation of isotopic, fluorescent, and heavy-atom-modified nucleotides into RNAs by position-selective labeling of RNA.,Liu Y, Holmstrom E, Yu P, Tan K, Zuo X, Nesbitt DJ, Sousa R, Stagno JR, Wang YX Nat Protoc. 2018 May;13(5):987-1005. doi: 10.1038/nprot.2018.002. Epub 2018 Apr, 12. PMID:29651055<ref>PMID:29651055</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5uza" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 05:53, 25 April 2018
Adenine riboswitch aptamer domain labelled with iodo-uridine by position-selective labelling of RNA (PLOR)
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