2f2j
From Proteopedia
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[[Image:2f2j.gif|left|200px]] | [[Image:2f2j.gif|left|200px]] | ||
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| - | + | {{STRUCTURE_2f2j| PDB=2f2j | SCENE= }} | |
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'''Solution structure of [W19K, P20N, V21K]-kalata B1''' | '''Solution structure of [W19K, P20N, V21K]-kalata B1''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2F2J is a [[Single protein]] structure | + | 2F2J is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F2J OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Craik, D J.]] | [[Category: Craik, D J.]] | ||
[[Category: Daly, N L.]] | [[Category: Daly, N L.]] | ||
| - | [[Category: | + | [[Category: Beta-hairpin]] |
| - | [[Category: | + | [[Category: Circular backbone]] |
| - | [[Category: | + | [[Category: Cystine knot]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:23:23 2008'' | |
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Revision as of 00:23, 4 May 2008
Solution structure of [W19K, P20N, V21K]-kalata B1
Overview
The cyclotide family of plant proteins is of interest because of their unique topology, which combines a head-to-tail cyclic backbone with an embedded cystine knot, and because their remarkable chemical and biological properties make them ideal candidates as grafting templates for biologically active peptide epitopes. The present study describes the first steps towards exploiting the cyclotide framework by synthesizing and structurally characterizing two grafted analogues of the cyclotide kalata B1. The modified peptides have polar or charged residues substituted for residues that form part of a surface-exposed hydrophobic patch that plays a significant role in the folding and biological activity of kalata B1. Both analogues retain the native cyclotide fold, but lack the undesired haemolytic activity of their parent molecule, kalata B1. This finding confirms the tolerance of the cyclotide framework to residue substitutions and opens up possibilities for the substitution of biologically active peptide epitopes into the framework.
About this Structure
2F2J is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Structural plasticity of the cyclic-cystine-knot framework: implications for biological activity and drug design., Clark RJ, Daly NL, Craik DJ, Biochem J. 2006 Feb 15;394(Pt 1):85-93. PMID:16300479 Page seeded by OCA on Sun May 4 03:23:23 2008
