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1r19

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Revision as of 13:16, 16 December 2020

Crystal Structure Analysis of S.epidermidis adhesin SdrG binding to Fibrinogen (Apo structure)

Structural highlights

1r19 is a 4 chain structure with sequence from "albococcus_epidermidis"_winslow_and_winslow_1908 "albococcus epidermidis" winslow and winslow 1908. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1r17
Gene:	sdrG ("Albococcus epidermidis" Winslow and Winslow 1908)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SDRG_STAEP] Binds to the N-terminus of the B beta-chain of human fibrinogen and thereby interferes with thrombin cleavage and release of fibrinopeptide B, thus interfering with fibrin clot formation. This may hinder host defense against microbial infections.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Gram-positive pathogens such as staphylococci contain multiple cell wall-anchored proteins that serve as an interface between the microbe and its environment. Some of these proteins act as adhesins and mediate bacterial attachment to host tissues. SdrG is a cell wall-anchored adhesin from Staphylococcus epidermidis that binds to the Bbeta chain of human fibrinogen (Fg) and is necessary and sufficient for bacterial attachment to Fg-coated biomaterials. Here, we present the crystal structures of the ligand binding region of SdrG as an apoprotein and in complex with a synthetic peptide analogous to its binding site in Fg. Analysis of the crystal structures, along with mutational studies of both the protein and of the peptide, reveals that SdrG binds to its ligand with a dynamic "dock, lock, and latch" mechanism. We propose that this mechanism represents a general mode of ligand binding for structurally related cell wall-anchored proteins of gram-positive bacteria.

A "dock, lock, and latch" structural model for a staphylococcal adhesin binding to fibrinogen.,Ponnuraj K, Bowden MG, Davis S, Gurusiddappa S, Moore D, Choe D, Xu Y, Hook M, Narayana SV Cell. 2003 Oct 17;115(2):217-28. PMID:14567919^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Davis SL, Gurusiddappa S, McCrea KW, Perkins S, Hook M. SdrG, a fibrinogen-binding bacterial adhesin of the microbial surface components recognizing adhesive matrix molecules subfamily from Staphylococcus epidermidis, targets the thrombin cleavage site in the Bbeta chain. J Biol Chem. 2001 Jul 27;276(30):27799-805. Epub 2001 May 22. PMID:11371571 doi:http://dx.doi.org/10.1074/jbc.M103873200
↑ Ponnuraj K, Bowden MG, Davis S, Gurusiddappa S, Moore D, Choe D, Xu Y, Hook M, Narayana SV. A "dock, lock, and latch" structural model for a staphylococcal adhesin binding to fibrinogen. Cell. 2003 Oct 17;115(2):217-28. PMID:14567919

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1r19"

@@ Line 1: / Line 1: @@
 ==Crystal Structure Analysis of S.epidermidis adhesin SdrG binding to Fibrinogen (Apo structure)==
-<StructureSection load='1r19' size='340' side='right' caption='[[1r19]], [[Resolution|resolution]] 3.51&Aring;' scene=''>
+<StructureSection load='1r19' size='340' side='right'caption='[[1r19]], [[Resolution|resolution]] 3.51&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1r19]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"albococcus_epidermidis"_winslow_and_winslow_1908 "albococcus epidermidis" winslow and winslow 1908]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R19 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1R19 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1r19]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"albococcus_epidermidis"_winslow_and_winslow_1908 "albococcus epidermidis" winslow and winslow 1908]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R19 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1R19 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1r17|1r17]]</td></tr>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1r17|1r17]]</div></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sdrG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1282 "Albococcus epidermidis" Winslow and Winslow 1908])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r19 OCA], [http://pdbe.org/1r19 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1r19 RCSB], [http://www.ebi.ac.uk/pdbsum/1r19 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1r19 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1r19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r19 OCA], [http://pdbe.org/1r19 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1r19 RCSB], [http://www.ebi.ac.uk/pdbsum/1r19 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1r19 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 37: / Line 37: @@
 </StructureSection>
 [[Category: Albococcus epidermidis winslow and winslow 1908]]
+[[Category: Large Structures]]
 [[Category: Bowden, M G]]
 [[Category: Choe, D]]

1r19

From Proteopedia

Revision as of 13:16, 16 December 2020

Crystal Structure Analysis of S.epidermidis adhesin SdrG binding to Fibrinogen (Apo structure)

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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