2f8k

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[[Image:2f8k.gif|left|200px]]
[[Image:2f8k.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2f8k |SIZE=350|CAPTION= <scene name='initialview01'>2f8k</scene>, resolution 2.000&Aring;
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The line below this paragraph, containing "STRUCTURE_2f8k", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= VTS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
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-->
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|DOMAIN=
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{{STRUCTURE_2f8k| PDB=2f8k | SCENE= }}
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|RELATEDENTRY=[[2d3d|2D3D]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8k OCA], [http://www.ebi.ac.uk/pdbsum/2f8k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2f8k RCSB]</span>
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}}
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'''Sequence specific recognition of RNA hairpins by the SAM domain of Vts1'''
'''Sequence specific recognition of RNA hairpins by the SAM domain of Vts1'''
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[[Category: Sicheri, F.]]
[[Category: Sicheri, F.]]
[[Category: Smibert, C A.]]
[[Category: Smibert, C A.]]
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[[Category: sam domain]]
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[[Category: Sam domain]]
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[[Category: vts1/rna complex]]
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[[Category: Vts1/rna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:35:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:59:23 2008''
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Revision as of 00:35, 4 May 2008

Image:2f8k.gif

Template:STRUCTURE 2f8k

Sequence specific recognition of RNA hairpins by the SAM domain of Vts1


Overview

The SAM domain of the Saccharomyces cerevisiae post-transcriptional regulator Vts1p epitomizes a subfamily of SAM domains conserved from yeast to humans that function as sequence-specific RNA-binding domains. Here we report the 2.0-A X-ray structure of the Vts1p SAM domain bound to a high-affinity RNA ligand. Specificity of RNA binding arises from the association of a guanosine loop base with a shallow pocket on the SAM domain and from multiple SAM domain contacts to the unique backbone structure of the loop, defined in part by a nonplanar base pair within the loop. We have validated NNF1 as an endogenous target of Vts1p among 79 transcripts that copurify with Vts1p. Bioinformatic analysis of these mRNAs demonstrates that the RNA-binding specificity of Vts1p in vivo is probably more stringent than that of the isolated SAM domain in vitro.

About this Structure

2F8K is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Sequence-specific recognition of RNA hairpins by the SAM domain of Vts1p., Aviv T, Lin Z, Ben-Ari G, Smibert CA, Sicheri F, Nat Struct Mol Biol. 2006 Feb;13(2):168-76. Epub 2006 Jan 22. PMID:16429151 Page seeded by OCA on Sun May 4 03:35:31 2008

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