2f8v
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2f8v.gif|left|200px]] | [[Image:2f8v.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2f8v", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2f8v| PDB=2f8v | SCENE= }} | |
| - | | | + | |
| - | + | ||
| - | }} | + | |
'''Structure of full length telethonin in complex with the N-terminus of titin''' | '''Structure of full length telethonin in complex with the N-terminus of titin''' | ||
| Line 35: | Line 32: | ||
[[Category: Wilmanns, M.]] | [[Category: Wilmanns, M.]] | ||
[[Category: Zou, P.]] | [[Category: Zou, P.]] | ||
| - | [[Category: | + | [[Category: Sarcomere]] |
| - | [[Category: | + | [[Category: Telethonin]] |
| - | [[Category: | + | [[Category: Titin]] |
| - | [[Category: | + | [[Category: Z1z2]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:36:10 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 00:36, 4 May 2008
Structure of full length telethonin in complex with the N-terminus of titin
Contents |
Overview
The Z-disk region defines the lateral boundary of the sarcomere and requires a high level of mechanical strength to provide a stable framework for large filamentous muscle proteins. The level of complexity at this area is reflected by a large number of protein-protein interactions. Recently, we unraveled how the N-terminus of the longest filament component, the giant muscle protein titin, is assembled into an antiparallel (2:1) sandwich complex by the N-terminal titin-binding segment of the Z-disk ligand telethonin/T-cap [Zou, P., Pinotsis, N., Lange, S., Song, Y.H., Popov, A., Mavridis, I., Mayans, O.M., Gautel, M., Wilmanns, M., 2006. Palindromic assembly of the giant muscle protein titin in the sarcomeric Z-disk. Nature 439, 229-233]. In this contribution, we present structural data of a related complex of the titin N-terminus with full-length telethonin. The C-terminus of telethonin remains invisible, suggesting that it does not fold into a defined structure even in the presence of titin. In contrast to the structure with truncated telethonin, a dimer of two titin/telethonin complexes is formed within the crystal environment, potentially indicating the formation of higher oligomers. We further investigated the structure and dynamics of this assembly by small-angle X-ray scattering, circular dichroism, and in vivo complementation data. The data consistently indicate the involvement of the C-terminal part of telethonin into the assembly of two titin/telethonin complexes.
Disease
Known disease associated with this structure: Cardiomyopathy, dilated, 1N OMIM:[604488], Muscular dystrophy, limb-girdle, type 2G OMIM:[604488]
About this Structure
2F8V is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Evidence for a dimeric assembly of two titin/telethonin complexes induced by the telethonin C-terminus., Pinotsis N, Petoukhov M, Lange S, Svergun D, Zou P, Gautel M, Wilmanns M, J Struct Biol. 2006 Aug;155(2):239-50. Epub 2006 Apr 27. PMID:16713295 Page seeded by OCA on Sun May 4 03:36:10 2008
Categories: Homo sapiens | Protein complex | Gautel, M. | Lange, S. | Petoukhov, M. | Pinotsis, N. | Svergun, D. | Wilmanns, M. | Zou, P. | Sarcomere | Telethonin | Titin | Z1z2
