6al7
From Proteopedia
(Difference between revisions)
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<StructureSection load='6al7' size='340' side='right' caption='[[6al7]], [[Resolution|resolution]] 1.69Å' scene=''> | <StructureSection load='6al7' size='340' side='right' caption='[[6al7]], [[Resolution|resolution]] 1.69Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6al7]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AL7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AL7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6al7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Fischerella_sp._atcc_43239 Fischerella sp. atcc 43239]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AL7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AL7 FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5wpp|5wpp]], [[5wpr|5wpr]], [[5wps|5wps]], [[5wpu|5wpu]], [[6al6|6al6]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5wpp|5wpp]], [[5wpr|5wpr]], [[5wps|5wps]], [[5wpu|5wpu]], [[6al6|6al6]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hpiU5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1535197 Fischerella sp. ATCC 43239])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6al7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6al7 OCA], [http://pdbe.org/6al7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6al7 RCSB], [http://www.ebi.ac.uk/pdbsum/6al7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6al7 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6al7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6al7 OCA], [http://pdbe.org/6al7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6al7 RCSB], [http://www.ebi.ac.uk/pdbsum/6al7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6al7 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Hapalindole alkaloids are a structurally diverse class of cyanobacterial natural products defined by their varied polycyclic ring systems and diverse biological activities. These complex metabolites are generated from a common biosynthetic intermediate by the Stig cyclases in three mechanistic steps: a rare Cope rearrangement, 6-exo-trig cyclization, and electrophilic aromatic substitution. Here we report the structure of HpiC1, a Stig cyclase that catalyzes the formation of 12-epi-hapalindole U in vitro. The 1.5-A structure revealed a dimeric assembly with two calcium ions per monomer and with the active sites located at the distal ends of the protein dimer. Mutational analysis and computational methods uncovered key residues for an acid-catalyzed [3,3]-sigmatropic rearrangement, as well as specific determinants that control the position of terminal electrophilic aromatic substitution, leading to a switch from hapalindole to fischerindole alkaloids. | ||
| + | |||
| + | Structural basis of the Cope rearrangement and cyclization in hapalindole biogenesis.,Newmister SA, Li S, Garcia-Borras M, Sanders JN, Yang S, Lowell AN, Yu F, Smith JL, Williams RM, Houk KN, Sherman DH Nat Chem Biol. 2018 Apr;14(4):345-351. doi: 10.1038/s41589-018-0003-x. Epub 2018 , Mar 12. PMID:29531360<ref>PMID:29531360</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6al7" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Fischerella sp. atcc 43239]] | ||
[[Category: Garcia-Borras, M]] | [[Category: Garcia-Borras, M]] | ||
[[Category: Houk, K N]] | [[Category: Houk, K N]] | ||
Revision as of 07:09, 28 March 2018
Crystal structure HpiC1 F138S
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