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| | ==STRUCTURAL BASIS FOR LIGHT ACITVATION OF A CHLOROPLAST ENZYME. THE STRUCTURE OF SORGHUM NADP-MALATE DEHYDROGENASE IN ITS OXIDIZED FORM== | | ==STRUCTURAL BASIS FOR LIGHT ACITVATION OF A CHLOROPLAST ENZYME. THE STRUCTURE OF SORGHUM NADP-MALATE DEHYDROGENASE IN ITS OXIDIZED FORM== |
| - | <StructureSection load='7mdh' size='340' side='right' caption='[[7mdh]], [[Resolution|resolution]] 2.40Å' scene=''> | + | <StructureSection load='7mdh' size='340' side='right'caption='[[7mdh]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[7mdh]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Andropogon_sorghum Andropogon sorghum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MDH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7MDH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7mdh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sorghum_bicolor Sorghum bicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MDH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MDH FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_dehydrogenase_(NADP(+)) Malate dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.82 1.1.1.82] </span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7mdh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mdh OCA], [http://pdbe.org/7mdh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7mdh RCSB], [http://www.ebi.ac.uk/pdbsum/7mdh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7mdh ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mdh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mdh OCA], [https://pdbe.org/7mdh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mdh RCSB], [https://www.ebi.ac.uk/pdbsum/7mdh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mdh ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MDHP1_SORBI MDHP1_SORBI]] The chloroplastic, NADP-dependent form is essential for the photosynthesis C4 cycle, which allows plants to circumvent the problem of photorespiration. In C4 plants, NADP-MDH activity acts to convert oxaloacetate to malate in chloroplasts of mesophyll cells for transport to the bundle sheath cells. | + | [https://www.uniprot.org/uniprot/MDHP1_SORBI MDHP1_SORBI] The chloroplastic, NADP-dependent form is essential for the photosynthesis C4 cycle, which allows plants to circumvent the problem of photorespiration. In C4 plants, NADP-MDH activity acts to convert oxaloacetate to malate in chloroplasts of mesophyll cells for transport to the bundle sheath cells. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Malate dehydrogenase|Malate dehydrogenase]] | + | *[[Malate Dehydrogenase 3D structures|Malate Dehydrogenase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Andropogon sorghum]] | + | [[Category: Large Structures]] |
| - | [[Category: Eklund, H]] | + | [[Category: Sorghum bicolor]] |
| - | [[Category: Issakidis-Bourguet, E]] | + | [[Category: Eklund H]] |
| - | [[Category: Johansson, K]] | + | [[Category: Issakidis-Bourguet E]] |
| - | [[Category: Lemaire-Chamley, M]] | + | [[Category: Johansson K]] |
| - | [[Category: Miginiac-Maslow, M]] | + | [[Category: Lemaire-Chamley M]] |
| - | [[Category: Ramaswamy, S]] | + | [[Category: Miginiac-Maslow M]] |
| - | [[Category: Saarinen, M]] | + | [[Category: Ramaswamy S]] |
| - | [[Category: Activated by light]]
| + | [[Category: Saarinen M]] |
| - | [[Category: Chloroplastic malate dehydrogenase]]
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| Structural highlights
Function
MDHP1_SORBI The chloroplastic, NADP-dependent form is essential for the photosynthesis C4 cycle, which allows plants to circumvent the problem of photorespiration. In C4 plants, NADP-MDH activity acts to convert oxaloacetate to malate in chloroplasts of mesophyll cells for transport to the bundle sheath cells.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Some key chloroplast enzymes are activated by light via a ferredoxin-thioredoxin reduction system which reduces disulfide bridges in the enzymes. We describe for the first time the structural basis for the redox activation of a chloroplast enzyme, the NADP-dependent malate dehydrogenase (MDH) from Sorghum vulgare whose structure has been determined and refined at 2.4 A resolution. In addition to the normal structural components of MDHs, the enzyme exhibits extensions at both the N- and C-termini, each of which contains a regulatory disulfide bridge which must be reduced for activation. The N-terminal disulfide motif is inserted in a cleft between the two subunits of the dimer, thereby locking the domains in each subunit. The C-terminal disulfide keeps the C-terminal residues tight to the enzyme surface and blocks access to the active site. Reduction of the N-terminal disulfide would release the stopper between the domains and give the enzyme the necessary flexibility. Simultaneous reduction of the C-terminal disulfide would free the C-terminal residues from binding to the enzyme and make the active site accessible.
Structural basis for light activation of a chloroplast enzyme: the structure of sorghum NADP-malate dehydrogenase in its oxidized form.,Johansson K, Ramaswamy S, Saarinen M, Lemaire-Chamley M, Issakidis-Bourguet E, Miginiac-Maslow M, Eklund H Biochemistry. 1999 Apr 6;38(14):4319-26. PMID:10194350[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Johansson K, Ramaswamy S, Saarinen M, Lemaire-Chamley M, Issakidis-Bourguet E, Miginiac-Maslow M, Eklund H. Structural basis for light activation of a chloroplast enzyme: the structure of sorghum NADP-malate dehydrogenase in its oxidized form. Biochemistry. 1999 Apr 6;38(14):4319-26. PMID:10194350 doi:10.1021/bi982876c
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