This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

1k3e

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 07:39, 25 November 2020

Type III secretion chaperone CesT

Structural highlights

1k3e is a 2 chain structure with sequence from Eco57. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	cesT (ECO57)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CEST_ECOLX] Chaperone for the type III secretion of Tir. Probably stabilizes the protein, prevents inappropriate protein-protein interactions and aids in secretion (By similarity).

Publication Abstract from PubMed

Several Gram-negative bacterial pathogens have evolved a type III secretion system to deliver virulence effector proteins directly into eukaryotic cells, a process essential for disease. This specialized secretion process requires customized chaperones specific for particular effector proteins. The crystal structures of the enterohemorrhagic Escherichia coli O157:H7 Tir-specific chaperone CesT and the Salmonella enterica SigD-specific chaperone SigE reveal a common overall fold and formation of homodimers. Site-directed mutagenesis suggests that variable, delocalized hydrophobic surfaces observed on the chaperone homodimers are responsible for specific binding to a particular effector protein. Isothermal titration calorimetry studies of Tir-CesT and enzymatic activity profiles of SigD-SigE indicate that the effector proteins are not globally unfolded in the presence of their cognate chaperones.

Structural and biochemical characterization of the type III secretion chaperones CesT and SigE.,Luo Y, Bertero MG, Frey EA, Pfuetzner RA, Wenk MR, Creagh L, Marcus SL, Lim D, Sicheri F, Kay C, Haynes C, Finlay BB, Strynadka NC Nat Struct Biol. 2001 Dec;8(12):1031-6. PMID:11685226^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Luo Y, Bertero MG, Frey EA, Pfuetzner RA, Wenk MR, Creagh L, Marcus SL, Lim D, Sicheri F, Kay C, Haynes C, Finlay BB, Strynadka NC. Structural and biochemical characterization of the type III secretion chaperones CesT and SigE. Nat Struct Biol. 2001 Dec;8(12):1031-6. PMID:11685226 doi:10.1038/nsb717

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1k3e"

@@ Line 1: / Line 1: @@
 ==Type III secretion chaperone CesT==
-<StructureSection load='1k3e' size='340' side='right' caption='[[1k3e]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+<StructureSection load='1k3e' size='340' side='right'caption='[[1k3e]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1k3e]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K3E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K3E FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1k3e]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K3E OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1K3E FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cesT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k3e OCA], [http://pdbe.org/1k3e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1k3e RCSB], [http://www.ebi.ac.uk/pdbsum/1k3e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1k3e ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1k3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k3e OCA], [http://pdbe.org/1k3e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1k3e RCSB], [http://www.ebi.ac.uk/pdbsum/1k3e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1k3e ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 23: / Line 23: @@
 </StructureSection>
 [[Category: Eco57]]
+[[Category: Large Structures]]
 [[Category: Bertero, M]]
 [[Category: Creagh, L]]

1k3e

From Proteopedia

Revision as of 07:39, 25 November 2020

Type III secretion chaperone CesT

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox