2fi2
From Proteopedia
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'''Solution structure of the SCAN homodimer from MZF-1/ZNF42''' | '''Solution structure of the SCAN homodimer from MZF-1/ZNF42''' | ||
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[[Category: Volkman, B F.]] | [[Category: Volkman, B F.]] | ||
[[Category: Waltner, J K.]] | [[Category: Waltner, J K.]] | ||
| - | [[Category: | + | [[Category: Center for eukaryotic structural genomic]] |
| - | [[Category: | + | [[Category: Cesg]] |
| - | [[Category: | + | [[Category: Homodimer]] |
| - | [[Category: | + | [[Category: Mzf-1]] |
| - | [[Category: | + | [[Category: Protein structure initiative]] |
| - | [[Category: | + | [[Category: Psi]] |
| - | [[Category: | + | [[Category: Scan domain]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | [[Category: | + | [[Category: Transcription factor]] |
| - | [[Category: | + | [[Category: Znf-42]] |
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Revision as of 00:55, 4 May 2008
Solution structure of the SCAN homodimer from MZF-1/ZNF42
Overview
The SCAN domain mediates interactions between members of a subfamily of zinc-finger transcription factors and is found in more than 60 C2H2 zinc finger genes in the human genome, including the tumor suppressor gene myeloid zinc finger 1 (MZF1). Glutathione-S-transferase pull-down assays showed that the MZF1 SCAN domain self-associates, and a Kd value of 600 nM was measured by intrinsic tryptophan fluorescence polarization. The MZF1 structure determined by NMR spectroscopy revealed a domain-swapped dimer. Each monomer consists of five alpha helices in two subdomains connected by the alpha2-alpha3 loop. Residues from helix 3 of each monomer compose the core of the dimer interface, while the alpha1-alpha2 loop and helix 2 pack against helices 3 and 5 from the opposing monomer. Comprehensive sequence analysis is coupled with the first high-resolution structure of a SCAN dimer to provide an initial view of the recognition elements that govern dimerization for this large family of transcription factors.
About this Structure
2FI2 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the SCAN domain from the tumor suppressor protein MZF1., Peterson FC, Hayes PL, Waltner JK, Heisner AK, Jensen DR, Sander TL, Volkman BF, J Mol Biol. 2006 Oct 13;363(1):137-47. Epub 2006 Jul 31. PMID:16950398 Page seeded by OCA on Sun May 4 03:55:28 2008
Categories: Homo sapiens | Single protein | CESG, Center for Eukaryotic Structural Genomics. | Peterson, F C. | Sander, T L. | Volkman, B F. | Waltner, J K. | Center for eukaryotic structural genomic | Cesg | Homodimer | Mzf-1 | Protein structure initiative | Psi | Scan domain | Structural genomic | Transcription factor | Znf-42
