This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

1se7

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 11:21, 24 December 2020

Solution structure of the E. coli bacteriophage P1 encoded HOT protein: a homologue of the theta subunit of E. coli DNA polymerase III

Structural highlights

1se7 is a 1 chain structure with sequence from Bpp1. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	HOT (BPP1)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

DNA polymerase III, the main replicative polymerase of E. coli, contains a small subunit, theta, that binds to the epsilon proofreading subunit and appears to enhance the enzyme's proofreading function--especially under extreme conditions. It was recently discovered that E. coli bacteriophage P1 encodes a theta homolog, named HOT. The (1)H-(15)N HSQC spectrum of HOT exhibits more uniform intensities and less evidence of conformational exchange than that of theta; this uniformity facilitates a determination of the HOT solution structure by NMR. The structure contains three alpha helices, as reported previously for theta; however, the folding topology of the two proteins is very different. Residual dipolar coupling measurements on labeled theta support the conclusion that it is structurally homologous with HOT. As judged by CD measurements, the melting temperature of HOT was 62 degrees C, compared to 56 degrees C for theta, consistent with other data suggesting greater thermal stability of the HOT protein.

Phage like it HOT: solution structure of the bacteriophage P1-encoded HOT protein, a homolog of the theta subunit of E. coli DNA polymerase III.,Derose EF, Kirby TW, Mueller GA, Chikova AK, Schaaper RM, London RE Structure. 2004 Dec;12(12):2221-31. PMID:15576035^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Derose EF, Kirby TW, Mueller GA, Chikova AK, Schaaper RM, London RE. Phage like it HOT: solution structure of the bacteriophage P1-encoded HOT protein, a homolog of the theta subunit of E. coli DNA polymerase III. Structure. 2004 Dec;12(12):2221-31. PMID:15576035 doi:10.1016/j.str.2004.09.019

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1se7"

@@ Line 1: / Line 1: @@
 ==Solution structure of the E. coli bacteriophage P1 encoded HOT protein: a homologue of the theta subunit of E. coli DNA polymerase III==
-<StructureSection load='1se7' size='340' side='right' caption='[[1se7]], [[NMR_Ensembles_of_Models | 7 NMR models]]' scene=''>
+<StructureSection load='1se7' size='340' side='right'caption='[[1se7]], [[NMR_Ensembles_of_Models | 7 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1se7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpp1 Bpp1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SE7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SE7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1se7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpp1 Bpp1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SE7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1SE7 FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HOT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10678 BPP1])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1se7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1se7 OCA], [http://pdbe.org/1se7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1se7 RCSB], [http://www.ebi.ac.uk/pdbsum/1se7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1se7 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1se7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1se7 OCA], [http://pdbe.org/1se7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1se7 RCSB], [http://www.ebi.ac.uk/pdbsum/1se7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1se7 ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 31: / Line 31: @@
 </StructureSection>
 [[Category: Bpp1]]
+[[Category: Large Structures]]
 [[Category: Chikova, A K]]
 [[Category: DeRose, E F]]

1se7

From Proteopedia

Revision as of 11:21, 24 December 2020

Solution structure of the E. coli bacteriophage P1 encoded HOT protein: a homologue of the theta subunit of E. coli DNA polymerase III

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox