1uzi
From Proteopedia
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| - | [[Image:1uzi. | + | [[Image:1uzi.jpg|left|200px]]<br /><applet load="1uzi" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1uzi" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1uzi, resolution 1.89Å" /> | caption="1uzi, resolution 1.89Å" /> | ||
'''C3 EXOENZYME FROM CLOSTRIDIUM BOTULINUM, TETRAGONAL FORM'''<br /> | '''C3 EXOENZYME FROM CLOSTRIDIUM BOTULINUM, TETRAGONAL FORM'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1UZI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum] with VO4, V4O and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/NAD(+)_ADP-ribosyltransferase NAD(+) ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.30 2.4.2.30] | + | 1UZI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum] with VO4, V4O and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/NAD(+)_ADP-ribosyltransferase NAD(+) ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.30 2.4.2.30] Known structural/functional Site: <scene name='pdbsite=AC1:Vo4 Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UZI OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:19:28 2007'' |
Revision as of 16:09, 18 December 2007
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C3 EXOENZYME FROM CLOSTRIDIUM BOTULINUM, TETRAGONAL FORM
Overview
C3 exoenzyme from Clostridium botulinum (C3bot1) ADP-ribosylates and, thereby inactivates Rho A, B and C GTPases in mammalian cells. The, structure of a tetragonal crystal form has been determined by molecular, replacement and refined to 1.89 A resolution. It is very similar to the, apo structures determined previously from two different monoclinic crystal, forms. An objective reassessment of available apo and nucleotide-bound, C3bot1 structures indicates that, contrary to a previous report, the, protein possesses a rigid core formed largely of beta-strands and that the, general flexure that accompanies NAD binding is concentrated in two, peripheral lobes. Tetragonal crystals disintegrate in the presence of NAD, most likely because of disruption of essential crystal contacts.
About this Structure
1UZI is a Single protein structure of sequence from Clostridium botulinum with VO4, V4O and GOL as ligands. Active as NAD(+) ADP-ribosyltransferase, with EC number 2.4.2.30 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
C3 exoenzyme from Clostridium botulinum: structure of a tetragonal crystal form and a reassessment of NAD-induced flexure., Evans HR, Holloway DE, Sutton JM, Ayriss J, Shone CC, Acharya KR, Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1502-5. Epub 2004, Jul 21. PMID:15272191
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