2fik

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[[Image:2fik.gif|left|200px]]
[[Image:2fik.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2fik |SIZE=350|CAPTION= <scene name='initialview01'>2fik</scene>, resolution 1.80&Aring;
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The line below this paragraph, containing "STRUCTURE_2fik", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=GSL:(2S,3R)-3-HYDROXY-2-(TETRADECANOYLAMINO)OCTADECYL+ALPHA-D-GALACTOPYRANOSIDURONIC+ACID'>GSL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= Cd1d1, Cd1.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]), beta-2-microglobulin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
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|DOMAIN=
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{{STRUCTURE_2fik| PDB=2fik | SCENE= }}
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|RELATEDENTRY=[[1z5l|1Z5L]], [[2akr|2AKR]], [[1zhn|1ZHN]], [[1zt4|1ZT4]], [[1cd1|1CD1]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fik FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fik OCA], [http://www.ebi.ac.uk/pdbsum/2fik PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fik RCSB]</span>
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}}
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'''Structure of a microbial glycosphingolipid bound to mouse CD1d'''
'''Structure of a microbial glycosphingolipid bound to mouse CD1d'''
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[[Category: Wu, D.]]
[[Category: Wu, D.]]
[[Category: Zajonc, D M.]]
[[Category: Zajonc, D M.]]
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[[Category: bacterial antigen]]
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[[Category: Bacterial antigen]]
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[[Category: cd1d]]
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[[Category: Cd1d]]
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[[Category: immune system]]
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[[Category: Immune system]]
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[[Category: mhc-fold]]
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[[Category: Mhc-fold]]
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[[Category: nkt cell]]
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[[Category: Nkt cell]]
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[[Category: tcr]]
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[[Category: Tcr]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:56:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:03:13 2008''
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Revision as of 00:56, 4 May 2008

Image:2fik.gif

Template:STRUCTURE 2fik

Structure of a microbial glycosphingolipid bound to mouse CD1d


Overview

Natural killer T (NKT) cells provide an innate-type immune response upon T cell receptor interaction with CD1d-presented antigens. We demonstrate through equilibrium tetramer binding and antigen presentation assays with Valpha14i-positive NKT cell hybridomas that the Sphingomonas glycolipid alpha-galacturonosyl ceramide (GalA-GSL) is a NKT cell agonist that is significantly weaker than alpha-galactosylceramide (alpha-GalCer), the most potent known NKT agonist. For GalA-GSL, a shorter fatty acyl chain, an absence of the 4-OH on the sphingosine tail and a 6'-COOH group on the galactose moiety account for its observed antigenic potency. We further determined the crystal structure of mCD1d in complex with GalA-GSL at 1.8-A resolution. The overall binding mode of GalA-GSL to mCD1d is similar to that of the short-chain alpha-GalCer ligand PBS-25, but its sphinganine chain is more deeply inserted into the F' pocket due to alternate hydrogen-bonding interactions between the sphinganine 3-OH with Asp-80. Subsequently, a slight lateral shift (>1 A) of the galacturonosyl head group is noted at the CD1 surface compared with the galactose of alpha-GalCer. Because the relatively short C(14) fatty acid of GalA-GSL does not fully occupy the A' pocket, a spacer lipid is found that stabilizes this pocket. The lipid spacer was identified by GC/MS as a mixture of saturated and monounsaturated palmitic acid (C(16)). Comparison of available crystal structures of alpha-anomeric glycosphingolipids now sheds light on the structural basis of their differential antigenic potency and has led to the design and synthesis of NKT cell agonists with enhanced cell-based stimulatory activities compared with alpha-GalCer.

About this Structure

2FIK is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

Reference

Design of natural killer T cell activators: structure and function of a microbial glycosphingolipid bound to mouse CD1d., Wu D, Zajonc DM, Fujio M, Sullivan BA, Kinjo Y, Kronenberg M, Wilson IA, Wong CH, Proc Natl Acad Sci U S A. 2006 Mar 14;103(11):3972-7. Epub 2006 Mar 6. PMID:16537470 Page seeded by OCA on Sun May 4 03:56:24 2008

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