6chr
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of a group II intron lariat with an intact 3' splice site (pre-2s state)== | |
| + | <StructureSection load='6chr' size='340' side='right'caption='[[6chr]], [[Resolution|resolution]] 3.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6chr]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CHR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CHR FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SPM:SPERMINE'>SPM</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6chr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6chr OCA], [http://pdbe.org/6chr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6chr RCSB], [http://www.ebi.ac.uk/pdbsum/6chr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6chr ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The group II intron and the spliceosome share a common active site architecture and are thought to be evolutionarily related. Here we report the 3.7 A crystal structure of a eukaryotic group II intron in the lariat-3' exon form, immediately preceding the second step of splicing, analogous to the spliceosomal P complex. This structure reveals the location of the intact 3' splice site within the catalytic core of the group II intron. The 3'-OH of the 5' exon is positioned in close proximity to the 3' splice site for nucleophilic attack and exon ligation. The active site undergoes conformational rearrangements with the catalytic triplex having different configurations before and after the second step of splicing. We describe a complete model for the second step of group II intron splicing that incorporates a dynamic catalytic triplex being responsible for creating the binding pocket for 3' splice site capture. | ||
| - | + | Structural basis for the second step of group II intron splicing.,Chan RT, Peters JK, Robart AR, Wiryaman T, Rajashankar KR, Toor N Nat Commun. 2018 Nov 8;9(1):4676. doi: 10.1038/s41467-018-06678-0. PMID:30410046<ref>PMID:30410046</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6chr" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Chan, R T]] | ||
| + | [[Category: Peters, J K]] | ||
| + | [[Category: Rajashankar, K R]] | ||
| + | [[Category: Robart, A R]] | ||
| + | [[Category: Toor, N]] | ||
| + | [[Category: Wiryaman, T]] | ||
| + | [[Category: Group ii intron]] | ||
| + | [[Category: Lariat 3'-exon]] | ||
| + | [[Category: Rna]] | ||
| + | [[Category: Rna splicing]] | ||
| + | [[Category: Spliceosome]] | ||
Revision as of 10:45, 1 January 2020
Crystal structure of a group II intron lariat with an intact 3' splice site (pre-2s state)
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