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2fkm

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[[Image:2fkm.gif|left|200px]]
[[Image:2fkm.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2fkm |SIZE=350|CAPTION= <scene name='initialview01'>2fkm</scene>, resolution 1.900&Aring;
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The line below this paragraph, containing "STRUCTURE_2fkm", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=G16:ALPHA-D-GLUCOSE+1,6-BISPHOSPHATE'>G16</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphomannomutase Phosphomannomutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.8 5.4.2.8] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= AlgC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])
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-->
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|DOMAIN=
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{{STRUCTURE_2fkm| PDB=2fkm | SCENE= }}
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|RELATEDENTRY=[[1k35|1K35]], [[1k2y|1K2Y]], [[1pcj|1PCJ]], [[1pcm|1PCM]], [[1p5d|1P5D]], [[1p5g|1P5G]], [[2fkf|2FKF]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fkm OCA], [http://www.ebi.ac.uk/pdbsum/2fkm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fkm RCSB]</span>
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}}
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'''PMM/PGM S108D mutant with alpha-d-glucose 1,6-bisphosphate bound'''
'''PMM/PGM S108D mutant with alpha-d-glucose 1,6-bisphosphate bound'''
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[[Category: Beamer, L J.]]
[[Category: Beamer, L J.]]
[[Category: Regni, C A.]]
[[Category: Regni, C A.]]
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[[Category: alpha/beta protein]]
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[[Category: Alpha/beta protein]]
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[[Category: enzyme-ligand complex]]
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[[Category: Enzyme-ligand complex]]
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[[Category: enzyme-metal complex]]
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[[Category: Enzyme-metal complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:00:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:04:08 2008''
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Revision as of 01:00, 4 May 2008

Image:2fkm.gif

Template:STRUCTURE 2fkm

PMM/PGM S108D mutant with alpha-d-glucose 1,6-bisphosphate bound


Overview

The enzyme phosphomannomutase/phosphoglucomutase (PMM/PGM) from Pseudomonas aeruginosa catalyzes the reversible conversion of 1-phospho to 6-phospho-sugars. The reaction entails two phosphoryl transfers, with an intervening 180 degrees reorientation of the reaction intermediate (e.g. glucose 1,6-bisphosphate) during catalysis. Reorientation of the intermediate occurs without dissociation from the active site of the enzyme and is, thus, a simple example of processivity, as defined by multiple rounds of catalysis without release of substrate. Structural characterization of two PMM/PGM-intermediate complexes with glucose 1,6-bisphosphate provides new insights into the reaction catalyzed by the enzyme, including the reorientation of the intermediate. Kinetic analyses of site-directed mutants prompted by the structural studies reveal active site residues critical for maintaining association with glucose 1,6-bisphosphate during its unique dynamic reorientation in the active site of PMM/PGM.

About this Structure

2FKM is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

Reference

The reaction of phosphohexomutase from Pseudomonas aeruginosa: structural insights into a simple processive enzyme., Regni C, Schramm AM, Beamer LJ, J Biol Chem. 2006 Jun 2;281(22):15564-71. Epub 2006 Apr 4. PMID:16595672 Page seeded by OCA on Sun May 4 04:00:29 2008

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