2fl1

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[[Image:2fl1.jpg|left|200px]]
[[Image:2fl1.jpg|left|200px]]
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{{Structure
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|PDB= 2fl1 |SIZE=350|CAPTION= <scene name='initialview01'>2fl1</scene>, resolution 2.4&Aring;
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The line below this paragraph, containing "STRUCTURE_2fl1", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XYG:[(4Z)-2-[(1Z)-ETHANIMIDOYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>XYG</scene>
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{{STRUCTURE_2fl1| PDB=2fl1 | SCENE= }}
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|RELATEDENTRY=[[1xa9|1XA9]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fl1 OCA], [http://www.ebi.ac.uk/pdbsum/2fl1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fl1 RCSB]</span>
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'''Crystal structure of red fluorescent protein from Zoanthus, zRFP574, at 2.4A resolution'''
'''Crystal structure of red fluorescent protein from Zoanthus, zRFP574, at 2.4A resolution'''
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[[Category: Popov, B.]]
[[Category: Popov, B.]]
[[Category: Tikhonova, T.]]
[[Category: Tikhonova, T.]]
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[[Category: beta barrel]]
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[[Category: Beta barrel]]
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[[Category: beta-can fold]]
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[[Category: Beta-can fold]]
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[[Category: button polyp]]
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[[Category: Button polyp]]
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[[Category: chromophore]]
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[[Category: Chromophore]]
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[[Category: crystal structure]]
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[[Category: Crystal structure]]
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[[Category: emission maximum 574nm]]
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[[Category: Emission maximum 574nm]]
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[[Category: fluorescent marker]]
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[[Category: Fluorescent marker]]
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[[Category: intersubunit interface]]
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[[Category: Intersubunit interface]]
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[[Category: red fluorescent protein]]
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[[Category: Red fluorescent protein]]
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[[Category: tightly packed tetramer]]
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[[Category: Tightly packed tetramer]]
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[[Category: zoanthus sp.]]
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[[Category: Zoanthus sp.]]
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[[Category: zrfp574]]
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[[Category: Zrfp574]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:00:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:04:12 2008''
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Revision as of 01:01, 4 May 2008

Image:2fl1.jpg

Template:STRUCTURE 2fl1

Crystal structure of red fluorescent protein from Zoanthus, zRFP574, at 2.4A resolution


Overview

The three-dimensional structure of the red fluorescent protein (RFP) zRFP574 from the button polyp Zoanthus sp. (two dimers per asymmetric unit, 231 x 4 amino acids) has been determined at 2.4 A resolution in space group C222(1). The crystal structure, refined to a crystallographic R factor of 0.203 (R(free) = 0.249), adopts the beta-barrel fold composed of 11 strands similar to that of the yellow fluorescent protein zYFP538. The zRFP574 chromophore, originating from the protein sequence Asp66-Tyr67-Gly68, has a two-ring structure typical of GFP-like proteins. The bond geometry of residue 66 shows the strong tendency of the corresponding C(alpha) atom to sp(2) hybridization as a consequence of N-acylimine bond formation. The zRFP574 chromophore contains the 65-66 cis-peptide bond characteristic of red fluorescent proteins. The chromophore phenolic ring adopts a cis conformation coplanar with the imidazolinone ring. The crystallographic study has revealed an unexpected chemical feature of the internal chromophore. A decarboxylated side chain of the chromophore-forming residue Asp66 has been observed in the structure. This additional post-translational modification is likely to play a key role in the bathochromic shift of the zRFP574 spectrum.

About this Structure

2FL1 is a Single protein structure of sequence from Zoanthus sp.. Full crystallographic information is available from OCA.

Reference

Structure of a red fluorescent protein from Zoanthus, zRFP574, reveals a novel chromophore., Pletneva N, Pletnev S, Tikhonova T, Popov V, Martynov V, Pletnev V, Acta Crystallogr D Biol Crystallogr. 2006 May;62(Pt 5):527-32. Epub 2006, Apr 19. PMID:16627946 Page seeded by OCA on Sun May 4 04:00:59 2008

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