6fx1
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Pholiota squarrosa lectin in complex with an octasaccharide== | |
| + | <StructureSection load='6fx1' size='340' side='right' caption='[[6fx1]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6fx1]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FX1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FX1 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=C4W:1-azido-beta-N-acetyl-D-glucosamine'>C4W</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=OOA:3-OXOOCTANOIC+ACID'>OOA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fx1 OCA], [http://pdbe.org/6fx1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fx1 RCSB], [http://www.ebi.ac.uk/pdbsum/6fx1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fx1 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We recombinantly produced and characterized the mini fungal lectin PhoSL. Despite a length of only 40 amino acids, PhoSL exclusively recognizes N-glycans with beta1,6-linked fucose. Core fucosylation influences the intrinsic properties and bioactivities of mammalian N-glycoproteins and its level is linked to various cancers. Thus, PhoSL serves as a promising tool for glycoprofiling. Without structural precedence, we solved its crystal structure using the zinc anomalous signal. We revealed an interlaced trimer creating a novel protein fold termed beta-prism III. Three biantennary core fucosylated N-glycan azides of 8 to 12 sugars were cocrystallized with PhoSL. The resulting highly resolved structures gave a detailed view on how the exclusive recognition of alpha1,6-fucosylated N-glycans by such a small protein occurs. This work also provided a protein consensus motif for the observed specificity as well as a glimpse on N-glycan flexibility upon binding. | ||
| - | + | Recognition of complex core fucosylated N-glycans by a mini lectin.,Cabanettes A, Perkams L, Spies C, Unverzagt C, Varrot A Angew Chem Int Ed Engl. 2018 Jun 29. doi: 10.1002/anie.201805165. PMID:29956878<ref>PMID:29956878</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| + | <div class="pdbe-citations 6fx1" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Cabanettes, A]] | [[Category: Cabanettes, A]] | ||
[[Category: Varrot, A]] | [[Category: Varrot, A]] | ||
| + | [[Category: Lectin]] | ||
| + | [[Category: Octasaccharide]] | ||
| + | [[Category: Sugar binding protein]] | ||
Revision as of 05:47, 11 July 2018
Crystal structure of Pholiota squarrosa lectin in complex with an octasaccharide
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