This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2fmj

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2fmj.gif|left|200px]]
[[Image:2fmj.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2fmj |SIZE=350|CAPTION= <scene name='initialview01'>2fmj</scene>, resolution 1.65&Aring;
+
The line below this paragraph, containing "STRUCTURE_2fmj", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE= sprT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1911 Streptomyces griseus])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2fmj| PDB=2fmj | SCENE= }}
-
|RELATEDENTRY=[[1sgt|1SGT]], [[1os8|1OS8]], [[1oss|1OSS]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fmj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fmj OCA], [http://www.ebi.ac.uk/pdbsum/2fmj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fmj RCSB]</span>
+
-
}}
+
'''220-loop mutant of streptomyces griseus trypsin'''
'''220-loop mutant of streptomyces griseus trypsin'''
Line 28: Line 25:
[[Category: Cera, E Di.]]
[[Category: Cera, E Di.]]
[[Category: Page, M J.]]
[[Category: Page, M J.]]
-
[[Category: serine protease]]
+
[[Category: Serine protease]]
-
[[Category: trypsin]]
+
[[Category: Trypsin]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:04:20 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:04:51 2008''
+

Revision as of 01:04, 4 May 2008

Image:2fmj.gif

Template:STRUCTURE 2fmj

220-loop mutant of streptomyces griseus trypsin


Overview

Serine proteases of the chymotrypsin family show a dichotomous amino acid distribution for residue 225. Enzymes carrying Tyr at position 225 are activated by Na(+), whereas those carrying Pro are devoid of Na(+) binding and activation. Previous studies have demonstrated that the Y225P conversion is sufficient to abrogate Na(+) activation in several enzymes. However, the reverse substitution P225Y is necessary but not sufficient to introduce Na(+) binding and activation. Here we report that Streptomyces griseus trypsin, carrying Pro-225, can be engineered into a Na(+)-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa. The findings represent the first instance of an engineered Na(+)-activated enzyme and a proof of principle that should enable the design of other proteases with enhanced catalytic activity and allosteric regulation mediated by monovalent cation binding.

About this Structure

2FMJ is a Single protein structure of sequence from Streptomyces griseus. Full crystallographic information is available from OCA.

Reference

Conversion of trypsin into a Na(+)-activated enzyme., Page MJ, Bleackley MR, Wong S, MacGillivray RT, Di Cera E, Biochemistry. 2006 Mar 7;45(9):2987-93. PMID:16503653 Page seeded by OCA on Sun May 4 04:04:20 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2fmj"
Personal tools