2fmx

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[[Image:2fmx.jpg|left|200px]]
[[Image:2fmx.jpg|left|200px]]
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{{Structure
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|PDB= 2fmx |SIZE=350|CAPTION= <scene name='initialview01'>2fmx</scene>, resolution 1.82&Aring;
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The line below this paragraph, containing "STRUCTURE_2fmx", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=GDP:GUANOSINE-5&#39;-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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{{STRUCTURE_2fmx| PDB=2fmx | SCENE= }}
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|RELATEDENTRY=[[2fa9|2FA9]], [[1f6b|1F6B]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fmx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fmx OCA], [http://www.ebi.ac.uk/pdbsum/2fmx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fmx RCSB]</span>
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'''An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg(2+)'''
'''An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg(2+)'''
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[[Category: Rao, Y.]]
[[Category: Rao, Y.]]
[[Category: Yuan, C.]]
[[Category: Yuan, C.]]
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[[Category: cop ii assembly]]
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[[Category: Cop ii assembly]]
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[[Category: crystal structure]]
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[[Category: Crystal structure]]
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[[Category: dimerization]]
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[[Category: Dimerization]]
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[[Category: sar1]]
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[[Category: Sar1]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:05:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:05:03 2008''
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Revision as of 01:05, 4 May 2008

Image:2fmx.jpg

Template:STRUCTURE 2fmx

An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg(2+)


Overview

Mg2+ is essential for guanosine triphosphatase activity and plays key roles in guanine nucleotide binding and preserving the structural integrity of GTP-binding proteins. To understand the structural basis for Mg2+ function during the GDP/GTP exchange process, we determined the crystal structure of Delta9-Sar1-GDP at low Mg2+ concentration at 1.8A. Two Sar1-GDP molecules in the crystal form a dimer with Mg2+ presenting only in molecule B but not in molecule A. The absence of Mg2+ induces significant conformational changes in the switch I region in molecule A that shows similarities with those of Ha-Ras bound to Sos. The current structure reveals an important regulatory role for Mg2+. We suggest that guanine nucleotide exchange factor may utilize this feature to generate an open conformation for GDP/GTP exchange. Furthermore, we propose a mechanism for COPII assembly and disassembly in which dimerization of Sar1 plays an important role.

About this Structure

2FMX is a Single protein structure of sequence from Cricetulus griseus. Full crystallographic information is available from OCA.

Reference

An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg2+., Rao Y, Bian C, Yuan C, Li Y, Chen L, Ye X, Huang Z, Huang M, Biochem Biophys Res Commun. 2006 Sep 29;348(3):908-15. Epub 2006 Aug 1. PMID:16899220 Page seeded by OCA on Sun May 4 04:05:04 2008

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