2fnq

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[[Image:2fnq.gif|left|200px]]
[[Image:2fnq.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2fnq |SIZE=350|CAPTION= <scene name='initialview01'>2fnq</scene>, resolution 3.200&Aring;
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The line below this paragraph, containing "STRUCTURE_2fnq", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Arachidonate_8-lipoxygenase Arachidonate 8-lipoxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.40 1.13.11.40] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_2fnq| PDB=2fnq | SCENE= }}
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|RELATEDENTRY=[[1u5u|1u5u]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fnq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fnq OCA], [http://www.ebi.ac.uk/pdbsum/2fnq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fnq RCSB]</span>
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}}
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'''Insights from the X-ray crystal structure of coral 8R-lipoxygenase: calcium activation via A C2-like domain and a structural basis of product chirality'''
'''Insights from the X-ray crystal structure of coral 8R-lipoxygenase: calcium activation via A C2-like domain and a structural basis of product chirality'''
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==About this Structure==
==About this Structure==
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2FNQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Plexaura_homomalla Plexaura homomalla]. This structure supersedes the now removed PDB entry 1ZQ4. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FNQ OCA].
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2FNQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Plexaura_homomalla Plexaura homomalla]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1zq4 1zq4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FNQ OCA].
==Reference==
==Reference==
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[[Category: Newcomer, M E.]]
[[Category: Newcomer, M E.]]
[[Category: Oldham, M L.]]
[[Category: Oldham, M L.]]
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[[Category: beta-barrel]]
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[[Category: Beta-barrel]]
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[[Category: c2-like domain]]
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[[Category: C2-like domain]]
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[[Category: eicosanoid]]
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[[Category: Eicosanoid]]
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[[Category: fatty acid]]
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[[Category: Fatty acid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:06:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:05:22 2008''
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Revision as of 01:06, 4 May 2008

Template:STRUCTURE 2fnq

Insights from the X-ray crystal structure of coral 8R-lipoxygenase: calcium activation via A C2-like domain and a structural basis of product chirality


Overview

Lipoxygenases (LOXs) catalyze the regio- and stereospecific dioxygenation of polyunsaturated membrane-embedded fatty acids. We report here the 3.2 A resolution structure of 8R-LOX from the Caribbean sea whip coral Plexaura homomalla, a LOX isozyme with calcium dependence and the uncommon R chiral stereospecificity. Structural and spectroscopic analyses demonstrated calcium binding in a C2-like membrane-binding domain, illuminating the function of similar amino acids in calcium-activated mammalian 5-LOX, the key enzyme in the pathway to the pro-inflammatory leukotrienes. Mutation of Ca(2+)-ligating amino acids in 8R-LOX resulted not only in a diminished capacity to bind membranes, as monitored by fluorescence resonance energy transfer, but also in an associated loss of Ca(2+)-regulated enzyme activity. Moreover, a structural basis for R chiral specificity is also revealed; creation of a small oxygen pocket next to Gly(428) (Ala in all S-LOX isozymes) promoted C-8 oxygenation with R chirality on the activated fatty acid substrate.

About this Structure

2FNQ is a Single protein structure of sequence from Plexaura homomalla. This structure supersedes the now removed PDB entry 1zq4. Full crystallographic information is available from OCA.

Reference

Insights from the X-ray crystal structure of coral 8R-lipoxygenase: calcium activation via a C2-like domain and a structural basis of product chirality., Oldham ML, Brash AR, Newcomer ME, J Biol Chem. 2005 Nov 25;280(47):39545-52. Epub 2005 Sep 14. PMID:16162493 Page seeded by OCA on Sun May 4 04:06:37 2008

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