1sqf

From Proteopedia

(Difference between revisions)

Revision as of 11:32, 24 December 2020

The crystal structure of E. coli Fmu binary complex with S-Adenosylmethionine at 2.1 A resolution

Structural highlights

1sqf is a 1 chain structure with sequence from "bacillus_coli"_migula_1895 "bacillus coli" migula 1895. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	SUN, FMU, FMV, RSMB, B3289 ("Bacillus coli" Migula 1895)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RSMB_ECOLI] Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The crystal structure of E. coli Fmu, determined at 1.65 A resolution for the apoenzyme and 2.1 A resolution in complex with AdoMet, is the first representative of the 5-methylcytosine RNA methyltransferase family that includes the human nucleolar proliferation-associated protein p120. Fmu contains three subdomains which share structural homology to DNA m(5)C methyltransferases and two RNA binding protein families. In the binary complex, the AdoMet cofactor is positioned within the active site near a novel arrangement of two conserved cysteines that function in cytosine methylation. The site is surrounded by a positively charged cleft large enough to bind its unique target stem loop within 16S rRNA. Docking of this stem loop RNA into the structure followed by molecular mechanics shows that the Fmu structure is consistent with binding to the folded RNA substrate.

The first structure of an RNA m5C methyltransferase, Fmu, provides insight into catalytic mechanism and specific binding of RNA substrate.,Foster PG, Nunes CR, Greene P, Moustakas D, Stroud RM Structure. 2003 Dec;11(12):1609-20. PMID:14656444^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gu XR, Gustafsson C, Ku J, Yu M, Santi DV. Identification of the 16S rRNA m5C967 methyltransferase from Escherichia coli. Biochemistry. 1999 Mar 30;38(13):4053-7. PMID:10194318 doi:http://dx.doi.org/10.1021/bi982364y
↑ Foster PG, Nunes CR, Greene P, Moustakas D, Stroud RM. The first structure of an RNA m5C methyltransferase, Fmu, provides insight into catalytic mechanism and specific binding of RNA substrate. Structure. 2003 Dec;11(12):1609-20. PMID:14656444

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1sqf"

@@ Line 1: / Line 1: @@
 ==The crystal structure of E. coli Fmu binary complex with S-Adenosylmethionine at 2.1 A resolution==
-<StructureSection load='1sqf' size='340' side='right' caption='[[1sqf]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+<StructureSection load='1sqf' size='340' side='right'caption='[[1sqf]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1sqf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SQF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SQF FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1sqf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SQF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1SQF FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SUN, FMU, FMV, RSMB, B3289 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sqf OCA], [http://pdbe.org/1sqf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sqf RCSB], [http://www.ebi.ac.uk/pdbsum/1sqf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1sqf ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1sqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sqf OCA], [http://pdbe.org/1sqf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sqf RCSB], [http://www.ebi.ac.uk/pdbsum/1sqf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1sqf ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 34: / Line 34: @@
 </StructureSection>
 [[Category: Bacillus coli migula 1895]]
+[[Category: Large Structures]]
 [[Category: Foster, P G]]
 [[Category: Greene, P]]

1sqf

From Proteopedia

Revision as of 11:32, 24 December 2020

The crystal structure of E. coli Fmu binary complex with S-Adenosylmethionine at 2.1 A resolution

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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