2fs2
From Proteopedia
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[[Image:2fs2.gif|left|200px]] | [[Image:2fs2.gif|left|200px]] | ||
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| - | + | {{STRUCTURE_2fs2| PDB=2fs2 | SCENE= }} | |
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'''Structure of the E. coli PaaI protein from the phyenylacetic acid degradation operon''' | '''Structure of the E. coli PaaI protein from the phyenylacetic acid degradation operon''' | ||
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[[Category: Solorzano, V.]] | [[Category: Solorzano, V.]] | ||
[[Category: Wu, J.]] | [[Category: Wu, J.]] | ||
| - | [[Category: | + | [[Category: Degradation]] |
| - | [[Category: | + | [[Category: New york structural genomix research consortium]] |
| - | [[Category: | + | [[Category: Nysgxrc]] |
| - | [[Category: | + | [[Category: Operon]] |
| - | [[Category: | + | [[Category: Phenylacetic acid]] |
| - | [[Category: | + | [[Category: Protein structure initiative]] |
| - | [[Category: | + | [[Category: Psi]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | [[Category: | + | [[Category: T820]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:14:42 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 01:14, 4 May 2008
Structure of the E. coli PaaI protein from the phyenylacetic acid degradation operon
Overview
The structure and biochemical function of the hot dog-fold thioesterase PaaI operative in the aerobic phenylacetate degradation pathway are examined. PaaI showed modest activity with phenylacetyl-coenzyme A, suggestive of a role in coenzyme A release from this pathway intermediate in the event of limiting downstream pathway enzymes. Minimal activity was observed with aliphatic acyl-coenzyme A thioesters, which ruled out PaaI function in the lower phenylacetate pathway. PaaI was most active with ring-hydroxylated phenylacetyl-coenzyme A thioesters. The x-ray crystal structure of the Escherichia coli thioesterase is reported and analyzed to define the structural basis of substrate recognition and catalysis. The contributions of catalytic and substrate binding residues, thus, identified were examined through steady-state kinetic analysis of site-directed mutant proteins.
About this Structure
2FS2 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structure, function, and mechanism of the phenylacetate pathway hot dog-fold thioesterase PaaI., Song F, Zhuang Z, Finci L, Dunaway-Mariano D, Kniewel R, Buglino JA, Solorzano V, Wu J, Lima CD, J Biol Chem. 2006 Apr 21;281(16):11028-38. Epub 2006 Feb 7. PMID:16464851 Page seeded by OCA on Sun May 4 04:14:42 2008
Categories: Escherichia coli | Single protein | Buglino, J A. | Burley, S K. | Kniewel, R. | Lima, C D. | NYSGXRC, New York Structural GenomiX Research Consortium. | Solorzano, V. | Wu, J. | Degradation | New york structural genomix research consortium | Nysgxrc | Operon | Phenylacetic acid | Protein structure initiative | Psi | Structural genomic | T820
