2ftn

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[[Image:2ftn.gif|left|200px]]
[[Image:2ftn.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2ftn |SIZE=350|CAPTION= <scene name='initialview01'>2ftn</scene>, resolution 1.60&Aring;
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The line below this paragraph, containing "STRUCTURE_2ftn", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CXM:N-CARBOXYMETHIONINE'>CXM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidylate_synthase Thymidylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.45 2.1.1.45] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= thyA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=
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{{STRUCTURE_2ftn| PDB=2ftn | SCENE= }}
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|RELATEDENTRY=[[2fto|2FTO]], [[2ftq|2FTQ]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ftn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ftn OCA], [http://www.ebi.ac.uk/pdbsum/2ftn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ftn RCSB]</span>
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}}
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'''E. coli thymidylate synthase Y94F mutant'''
'''E. coli thymidylate synthase Y94F mutant'''
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[[Category: Montfort, W R.]]
[[Category: Montfort, W R.]]
[[Category: Roberts, S A.]]
[[Category: Roberts, S A.]]
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[[Category: methyltransferase]]
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[[Category: Methyltransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:17:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:07:32 2008''
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Revision as of 01:17, 4 May 2008

Image:2ftn.gif

Template:STRUCTURE 2ftn

E. coli thymidylate synthase Y94F mutant


Overview

Tyr94 of Escherichia coli thymidylate synthase is thought to be involved, either directly or by activation of a water molecule, in the abstraction of a proton from C5 of the 2'-deoxyuridine 5'-monophosphate (dUMP) substrate. Mutation of Tyr94 leads to a 400-fold loss in catalytic activity. The structure of the Y94F mutant has been determined in the native state and as a ternary complex with thymidine 5'-monophosphate (dTMP) and 10-propargyl 5,8-dideazafolate (PDDF). There are no structural changes ascribable to the mutation other than loss of a water molecule hydrogen bonded to the tyrosine OH, which is consistent with a catalytic role for the phenolic OH.

About this Structure

2FTN is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure of the Y94F mutant of Escherichia coli thymidylate synthase., Roberts SA, Hyatt DC, Honts JE, Changchien L, Maley GF, Maley F, Montfort WR, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Sep 1;62(Pt, 9):840-3. Epub 2006 Aug 18. PMID:16946460 Page seeded by OCA on Sun May 4 04:17:50 2008

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