This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

5wna

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 07:13, 7 October 2020

Structure of antibody 3D3 bound to the central conserved region of RSV G

Structural highlights

5wna is a 6 chain structure with sequence from Hrsva and Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[GLYC_HRSVA] Attaches the virion to the host cell membrane by interacting with heparan sulfate, initiating the infection. Interacts with host CX3CR1, the receptor for the CX3C chemokine fractalkine, to modulate the immune response and facilitate infection. Unlike the other paramyxovirus attachment proteins, lacks both neuraminidase and hemagglutinating activities. Secreted glycoprotein G helps RSV escape antibody-dependent restriction of replication by acting as an antigen decoy and by modulating the activity of leukocytes bearing Fcgamma receptors.

Publication Abstract from PubMed

Respiratory syncytial virus (RSV) is a top cause of severe lower respiratory tract disease and mortality in young children and the elderly. The viral envelope G glycoprotein contributes to pathogenesis through its roles in host cell attachment and modulation of host immunity. Although the G glycoprotein is a target of protective RSV-neutralizing antibodies, its development as a vaccine antigen has been hindered by its heterogeneous glycosylation and sequence variability outside a conserved central domain (CCD). We describe the cocrystal structures of two high-affinity broadly neutralizing human monoclonal antibodies bound to the RSV G CCD. The antibodies bind to neighboring conformational epitopes, which we named antigenic sites gamma1 and gamma2, that span a highly conserved surface, illuminating an important region of vulnerability. We further show that isolated RSV G CCD activates the chemokine receptor CX3CR1 and that antibodies block this activity. These studies provide a template for rational vaccine design targeting this key contributor to RSV disease.

Structures of respiratory syncytial virus G antigen bound to broadly neutralizing antibodies.,Fedechkin SO, George NL, Wolff JT, Kauvar LM, DuBois RM Sci Immunol. 2018 Mar 9;3(21). pii: 3/21/eaar3534. doi:, 10.1126/sciimmunol.aar3534. Epub 2018 Mar 9. PMID:29523582^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Fedechkin SO, George NL, Wolff JT, Kauvar LM, DuBois RM. Structures of respiratory syncytial virus G antigen bound to broadly neutralizing antibodies. Sci Immunol. 2018 Mar 9;3(21). pii: 3/21/eaar3534. doi:, 10.1126/sciimmunol.aar3534. Epub 2018 Mar 9. PMID:29523582 doi:http://dx.doi.org/10.1126/sciimmunol.aar3534

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5wna"

@@ Line 1: / Line 1: @@
 ==Structure of antibody 3D3 bound to the central conserved region of RSV G==
-<StructureSection load='5wna' size='340' side='right' caption='[[5wna]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
+<StructureSection load='5wna' size='340' side='right'caption='[[5wna]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5wna]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Hrsva Hrsva] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WNA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WNA FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5wna]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Hrsva Hrsva] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WNA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WNA FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wna FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wna OCA], [http://pdbe.org/5wna PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wna RCSB], [http://www.ebi.ac.uk/pdbsum/5wna PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wna ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5wna FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wna OCA], [http://pdbe.org/5wna PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wna RCSB], [http://www.ebi.ac.uk/pdbsum/5wna PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wna ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 23: / Line 23: @@
 [[Category: Hrsva]]
 [[Category: Human]]
+[[Category: Large Structures]]
 [[Category: DuBois, R M]]
 [[Category: Fedechkin, S O]]

5wna

From Proteopedia

Revision as of 07:13, 7 October 2020

Structure of antibody 3D3 bound to the central conserved region of RSV G

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox