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Avidin
From Proteopedia
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| - | The binding affinity of biotin for the avidin <scene name='41/410356/Cv/10'>receptors</scene> is very hight or in other words, the strength of the interations between the biotin and the avidin receptors is so strong that washing the biotin-avidin complex is not enough to remove the ligand from its pocket. This is not even possible by adding more biotin molecules to the system avidin-biotin. Once a | + | The binding affinity of biotin for the avidin <scene name='41/410356/Cv/10'>receptors</scene> is very hight or in other words, the strength of the interations between the biotin and the avidin receptors is so strong that washing the biotin-avidin complex is not enough to remove the ligand from its pocket. This is not even possible by adding more biotin molecules to the system avidin-biotin. Once a biotin has bound a pocket in the avidin, it is almost imposible to remove it in a biological system! |
Each monomer is an eight-stranded antiparallel <scene name='41/410356/Cv/13'>beta-barrel</scene>. Binding of biotin involves a highly stabilized network of polar and hydrophobic interactions. | Each monomer is an eight-stranded antiparallel <scene name='41/410356/Cv/13'>beta-barrel</scene>. Binding of biotin involves a highly stabilized network of polar and hydrophobic interactions. | ||
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Unexpectedly, a residual N-acetylglucosamine moiety was detected in the deglycosylated avidin monomer. These <scene name='41/410356/Cv/14'>sugars</scene> appear along with the biotin but outside of the biotin binding pockets. | Unexpectedly, a residual N-acetylglucosamine moiety was detected in the deglycosylated avidin monomer. These <scene name='41/410356/Cv/14'>sugars</scene> appear along with the biotin but outside of the biotin binding pockets. | ||
| - | + | *<scene name='41/410356/Cv/12'>Biotin has bound a pocket in the avidin</scene>. | |
See also:<br /> | See also:<br /> | ||
* [[Molecular Playground/Streptavidin]] | * [[Molecular Playground/Streptavidin]] | ||
Revision as of 10:47, 15 May 2018
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3D structures of Avidin
Updated on 15-May-2018
Reference
- Livnah O, Bayer EA, Wilchek M, Sussman JL. Three-dimensional structures of avidin and the avidin-biotin complex. Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5076-80. PMID:8506353
- Livnah O, Bayer EA, Wilchek M, Sussman JL. The structure of the complex between avidin and the dye, 2-(4'-hydroxyazobenzene) benzoic acid (HABA). FEBS Lett. 1993 Aug 9;328(1-2):165-8. PMID:8344421
Proteopedia Page Contributors and Editors (what is this?)
Michal Harel, Alexander Berchansky, Joel L. Sussman, Marcin Jozef Suskiewicz, Jaime Prilusky
