This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2g23
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2g23.gif|left|200px]] | [[Image:2g23.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2g23", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2g23| PDB=2g23 | SCENE= }} | |
| - | | | + | |
| - | | | + | |
| - | }} | + | |
'''The crystal structure of hexameric phenoxazinone synthase''' | '''The crystal structure of hexameric phenoxazinone synthase''' | ||
| Line 30: | Line 27: | ||
[[Category: Smith, A W.]] | [[Category: Smith, A W.]] | ||
[[Category: Wang, M.]] | [[Category: Wang, M.]] | ||
| - | [[Category: | + | [[Category: Copper]] |
| - | [[Category: | + | [[Category: Hexamer]] |
| - | [[Category: | + | [[Category: Laccase]] |
| - | [[Category: | + | [[Category: Metalloprotein]] |
| - | [[Category: | + | [[Category: Multicopper oxidase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:36:31 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 01:36, 4 May 2008
The crystal structure of hexameric phenoxazinone synthase
Overview
The multicopper oxidase phenoxazinone synthase (PHS) catalyzes the penultimate step in the biosynthesis of the antibiotic actinomycin D by Streptomyces antibioticus. PHS exists in two oligomeric forms: a dimeric form and a hexameric form, with older actinomycin-producing cultures containing predominately the hexameric form. The structure of hexameric PHS has been determined using X-ray diffraction to a resolution limit of 2.30 A and is found to contain several unexpected and distinctive features. The structure forms a hexameric ring that is centered on a pseudo 6-fold axis and has an outer diameter of 185 A with a large central cavity that has a diameter of 50 A. This hexameric structure is stabilized by a long loop connecting two domains; bound to this long loop is a fifth copper atom that is present as a type 2 copper. This copper atom is not present in any other multicopper oxidase, and its presence appears to stabilize the hexameric structure.
About this Structure
2G23 is a Single protein structure of sequence from Streptomyces antibioticus. Full crystallographic information is available from OCA.
Reference
Structure of phenoxazinone synthase from Streptomyces antibioticus reveals a new type 2 copper center., Smith AW, Camara-Artigas A, Wang M, Allen JP, Francisco WA, Biochemistry. 2006 Apr 11;45(14):4378-87. PMID:16584173 Page seeded by OCA on Sun May 4 04:36:31 2008
