2g46

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[[Image:2g46.gif|left|200px]]
[[Image:2g46.gif|left|200px]]
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{{Structure
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|PDB= 2g46 |SIZE=350|CAPTION= <scene name='initialview01'>2g46</scene>
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The line below this paragraph, containing "STRUCTURE_2g46", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=MLZ:N-METHYL-LYSINE'>MLZ</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>
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|GENE= A612L ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10506 Paramecium bursaria Chlorella virus 1])
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|DOMAIN=
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{{STRUCTURE_2g46| PDB=2g46 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g46 OCA], [http://www.ebi.ac.uk/pdbsum/2g46 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2g46 RCSB]</span>
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'''structure of vSET in complex with meK27 H3 Pept. and cofactor product SAH'''
'''structure of vSET in complex with meK27 H3 Pept. and cofactor product SAH'''
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[[Category: Zheng, L.]]
[[Category: Zheng, L.]]
[[Category: Zhou, M M.]]
[[Category: Zhou, M M.]]
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[[Category: hsitone methyltransferase]]
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[[Category: Hsitone methyltransferase]]
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[[Category: nmr]]
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[[Category: Nmr]]
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[[Category: vset structure]]
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[[Category: Vset structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:40:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:11:38 2008''
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Revision as of 01:40, 4 May 2008

Image:2g46.gif

Template:STRUCTURE 2g46

structure of vSET in complex with meK27 H3 Pept. and cofactor product SAH


Overview

SET domain lysine methyltransferases are known to catalyze site and state-specific methylation of lysine residues in histones that is fundamental in epigenetic regulation of gene activation and silencing in eukaryotic organisms. Here we report the three-dimensional solution structure of the SET domain histone lysine methyltransferase (vSET) from Paramecium bursaria chlorella virus 1 bound to cofactor S-adenosyl-L-homocysteine and a histone H3 peptide containing mono-methylated lysine 27. The dimeric structure, mimicking an enzyme/cofactor/substrate complex, yields the structural basis of the substrate specificity and methylation multiplicity of the enzyme. Our results from mutagenesis and enzyme kinetics analyses argue that a general base mechanism is less likely for lysine methylation by SET domains; and that the only invariant active site residue tyrosine 105 in vSET facilitates methyl transfer from cofactor to the substrate lysine by aligning intermolecular interactions in the lysine access channel of the enzyme.

About this Structure

2G46 is a Single protein structure of sequence from Paramecium bursaria chlorella virus 1. Full crystallographic information is available from OCA.

Reference

Structural insights of the specificity and catalysis of a viral histone H3 lysine 27 methyltransferase., Qian C, Wang X, Manzur K, Sachchidanand, Farooq A, Zeng L, Wang R, Zhou MM, J Mol Biol. 2006 May 26;359(1):86-96. Epub 2006 Mar 20. PMID:16603186 Page seeded by OCA on Sun May 4 04:40:17 2008

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